Fernandez H L, Patterson M R, Duell M J
J Neurobiol. 1980 Nov;11(6):557-70. doi: 10.1002/neu.480110606.
The effects of rat obturator nerve extracts on total and 16S acetylcholinesterase (AChE) activity were studied in endplate regions of denervated anterior gracilis muscles maintained in organ culture for 48 hr. The decrease of total AChE activity in cultured muscles was similar to that observed in denervated muscles in vivo. This decrease in activity was partly prevented by addition of either 100 or 200 microliters nerve extract (2.7 mg/ml protein) to the nutrient medium. Nerve extract treatment also decreased the release of AChE activity from the muscle into the bathing medium. Conversely, rat serum (20 microliters: 90 mg/ml protein) had no effect on total AChE activity in muscle endplates, nor on release of the enzyme by the muscle. The 16S form of AChE was confined to motor endplate muscle regions and its activity was drastically decreased by denervation in both organ culture and in vivo preparations in a comparable manner. Nerve-extract supplemented cultures contained a significantly (p < 0.001) larger amount of endplate 16S AChE activity (140--145%) than the corresponding controls (100%). Our results suggest that some nerve soluble substance, other than serum contaminants or 16S AChE itself, affects the maintenance of 16S AChE at the neuromuscular junction.
研究了大鼠闭孔神经提取物对在器官培养中维持48小时的失神经支配的股薄肌前肌终板区域总乙酰胆碱酯酶（AChE）活性和16S AChE活性的影响。培养肌肉中总AChE活性的降低与体内失神经支配肌肉中观察到的情况相似。向营养培养基中添加100或200微升神经提取物（2.7毫克/毫升蛋白质）可部分阻止这种活性降低。神经提取物处理还减少了AChE活性从肌肉释放到浴液培养基中。相反，大鼠血清（20微升：90毫克/毫升蛋白质）对肌肉终板中的总AChE活性以及肌肉释放该酶均无影响。AChE的16S形式局限于运动终板肌肉区域，在器官培养和体内制剂中，其活性均因去神经支配而以类似方式急剧降低。添加神经提取物的培养物中终板16S AChE活性（140 - 145%）显著（p < 0.001）高于相应对照（100%）。我们的结果表明，除血清污染物或16S AChE本身外，某些神经可溶性物质会影响神经肌肉接头处16S AChE的维持。
