清醒大鼠的血脑葡萄糖转运：静脉注射与颈内动脉注射方法的比较。

Blood-brain glucose transport in the conscious rat: comparison of the intravenous and intracarotid injection methods.

作者信息

Gjedde A, Rasmussen M

出版信息

J Neurochem. 1980 Dec;35(6):1375-81. doi: 10.1111/j.1471-4159.1980.tb09012.x.

DOI:10.1111/j.1471-4159.1980.tb09012.x

Abstract

The unidirectioal transfer of D-glucose from blood to parietal cortex tissue of the brain of awake rats was measured by single intravenous injection of tracer glucose, as well as by single intracarotid injection according to the method of Oldendorf. The maximal unidirectional blood-brain glucose transfer rate (Tmax) was 407 mumol (100 g)-1 min-1 when measured by intravenous injection, and 352 mumol (100 g)-1 min-1 when measured by intracarotid injection. The half-saturation constants (Km) were 7.8 mM and 16.8 mM, respectively. The comparison shows that the two methods give similar results when cerebral perfusion is assessed accurately.

摘要

通过单次静脉注射示踪葡萄糖以及按照奥尔登多夫的方法进行单次颈内动脉注射，测量清醒大鼠大脑顶叶皮质组织中D -葡萄糖从血液的单向转运。通过静脉注射测量时，最大单向血脑葡萄糖转运速率（Tmax）为407 μmol（100 g）-1 min-1，通过颈内动脉注射测量时为352 μmol（100 g）-1 min-1。半饱和常数（Km）分别为7.8 mM和16.8 mM。比较表明，当准确评估脑灌注时，两种方法得出的结果相似。