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氘标记的顺式和反式-12-十八碳烯酸在人血浆和脂蛋白脂质中的分布。

Distribution of deuterium-labeled cis- and trans-12-octadecenoic acids in human plasma and lipoprotein lipids.

作者信息

Emken E A, Dutton H J, Rohwedder W K, Rakoff H, Adlof R O

出版信息

Lipids. 1980 Oct;15(10):864-71. doi: 10.1007/BF02534378.

DOI:10.1007/BF02534378
PMID:7442476
Abstract

Triglycerides containing cis- and trans-12-octadecenoic acid (12c-18:1 and 12t-18:1) and cis-9-octadecenoic acid (9c-18:1) labeled with deuterium were fed to 2 young adult male subjects. These fatty isomers each contained a different number of deuterium lables, which allowed mass spectrometric analysis to distinguish among them after they were fed as a mixture. This approach results in a direct comparison of the absorption and distribution of these 3 monenoic acids into blood plasma and lipoprotein lipids. Plasma lipid data indicated that all phospholipid fractions selectively incorporate 12c-18:1 and 12t-18:1 in preference to 9c-18:1. Discrimination against 12c-18:1 and 12t-18:1 compared to 9c-18:1 was found in the plasma neutral lipids, with a strong discrimination against 12t-18:1 incorporation into the cholesteryl ester fraction. Considerable reduction in the percentage of linoleic and arachidonic acid was observed when 12-18:1 isomers were incorporated in plasma triglyceride, phosphatidylcholine and spingomyelin samples. Chylomicron lipid analyses indicated that all isomers were well absorbed. Variation was observed in the relative distribution of 12c-18:1, 12t-18:1 and 9c-18:1 between the very low density, low density and high density lipoprotein lipid classes. No desaturation of 12c-18:1 to linoleic acid was detected.

摘要

将含有顺式和反式-12-十八碳烯酸（12c-18:1和12t-18:1）以及氘标记的顺式-9-十八碳烯酸（9c-18:1）的甘油三酯喂给2名年轻成年男性受试者。这些脂肪酸异构体各自含有不同数量的氘标记，这使得在将它们作为混合物喂食后，通过质谱分析能够区分它们。这种方法可以直接比较这三种单烯酸在血浆和脂蛋白脂质中的吸收和分布情况。血浆脂质数据表明，所有磷脂组分都优先选择性地掺入12c-18:1和12t-18:1，而不是9c-18:1。在血浆中性脂质中发现对12c-18:1和12t-18:1的区分，相对于9c-18:1，对12t-18:1掺入胆固醇酯组分有强烈的区分。当12-18:1异构体掺入血浆甘油三酯、磷脂酰胆碱和鞘磷脂样品中时，观察到亚油酸和花生四烯酸的百分比有相当大的降低。乳糜微粒脂质分析表明，所有异构体都被很好地吸收。在极低密度、低密度和高密度脂蛋白脂质类别之间，观察到12c-18:1、12t-18:1和9c-18:1的相对分布存在差异。未检测到12c-18:1去饱和为亚油酸的情况。

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