Application of atomic absorption spectrometry for the study of the subretinal fluid.

A method of determining the inorganic constituents of the subretinal fluid using atomic absorption spectrometry is presented. Due to the small volumes available of the samples, the addition of diluents was necessary in order to be able to carry out an analysis of the elements at the most sensitive wavelength in the optimal range of the calibration curve. Details concerning standard solutions, laboratory equipment, sample preparation, precision and accuracy are also considered.