Shabarova Z A, Dolinnaya N G, Turkin S I, Gromova E S
Nucleic Acids Res. 1980 Jun 11;8(11):2413-29. doi: 10.1093/nar/8.11.2413.
A new class of synthetic DNA duplexes containing repeating oligonucleotide sequences, double-helical concatemers, is characterized. The UV-absorption and circular dichroism of a concatemer formed in self-association of d(T-G-C-A-C-A-T-G) have been studied. The thermodynamical parameters of complex formation are the following: delta Ho1 = -9.2 +/- 0.3 kcal/mol, delta So1 = -27 +/- 1 e.u. The data obtained show that pseudopolymeric duplexes having structures that are similar to DNA-B-type helices are formed in solutions of d(T-G-C-A-C-A-T-G). Polymerization of 32P-d(pT-G-C-A-C-A-T-G) induced by water-soluble carbodiimide has been carried out under the conditions of concatemer stability. The yield of the dimer, a 16-member oligonucleotide, was 13%.
一类包含重复寡核苷酸序列的新型合成DNA双链体，即双螺旋串联体，已得到表征。对由d(T-G-C-A-C-A-T-G)自缔合形成的串联体的紫外吸收和圆二色性进行了研究。复合物形成的热力学参数如下：ΔH°1 = -9.2 ± 0.3千卡/摩尔，ΔS°1 = -27 ± 1熵单位。所得数据表明，在d(T-G-C-A-C-A-T-G)溶液中形成了具有类似于DNA-B型螺旋结构的假多聚双链体。在串联体稳定的条件下，通过水溶性碳二亚胺诱导32P-d(pT-G-C-A-C-A-T-G)进行聚合。二聚体（一种16聚体寡核苷酸）的产率为13%。
