薄膜中A-DNA的一种新的构象特异性红外波段。
A new conformation-specific infrared band of A-DNA in films.
作者信息
Pohle W, Fritzsche H
出版信息
Nucleic Acids Res. 1980 Jun 11;8(11):2527-35. doi: 10.1093/nar/8.11.2527.
Abstract
A band at 1185 cm-1 occurs in the infrared spectra of nucleic acids in their A-type conformation which has been reproduced in the literature, too. The absorbance of this band is proportional to the fraction of the A form of DNA samples containing a mixture of different forms. The 1185 cm-1 band has been assigned tentatively to a vibration of the sugar-phosphate backbone with a fairly high contribution from the sugar moiety. Drastic dehydration of the DNA films is accompanied by a continuous intensity decrease of the 1185 cm-1 band indicating a collapse of the A form.
摘要
在核酸的 A 型构象的红外光谱中出现了一个位于 1185 cm⁻¹ 的吸收带，该吸收带在文献中也有报道。这个吸收带的吸光度与含有不同构象混合物的 DNA 样品中 A 型构象的比例成正比。1185 cm⁻¹ 处的吸收带被初步归属于糖 - 磷酸主链的振动，其中糖部分的贡献相当大。DNA 薄膜的剧烈脱水伴随着 1185 cm⁻¹ 吸收带强度的持续下降，这表明 A 型构象的崩塌。
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本文引用的文献
1
A preliminay investigation on the molecular structure of rice dwarf virus ribonucleic acid.水稻矮缩病毒核糖核酸分子结构的初步研究。 （注：原英文文本中“preliminay”拼写错误，正确应为“preliminary”）
J Mol Biol. 1966 Mar;16(1):180-90. doi: 10.1016/s0022-2836(66)80271-1.
2
Infrared spectrum of a DNA-RNA hybrid.DNA-RNA杂交体的红外光谱。
Biopolymers. 1969;7(6):909-16. doi: 10.1002/bip.1969.360070606.
3
Optimised parameters for A-DNA and B-DNA.A-DNA和B-DNA的优化参数。
Biochem Biophys Res Commun. 1972 Jun 28;47(6):1504-9. doi: 10.1016/0006-291X(72)90243-4.
4
Raman spectral studies of nucleic acids. 3. Laser-excited spectra of ribosomal RNA.核酸的拉曼光谱研究。3. 核糖体RNA的激光激发光谱。
Biochim Biophys Acta. 1970 Aug 8;213(2):417-23. doi: 10.1016/0005-2787(70)90049-3.
5
New possibilities of investigating nucleic acids and nucleoproteins in aqueous solutions by infrared spectroscopy.通过红外光谱研究水溶液中核酸和核蛋白的新可能性。
Nature. 1972 Feb 18;235(5338):386-8. doi: 10.1038/235386a0.
6
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7
Direct evidence of the C-like form of sodium deoxyribonucleate.脱氧核糖酸钠C样形态的直接证据。
Proc Natl Acad Sci U S A. 1973 Dec;70(12):3352-5. doi: 10.1073/pnas.70.12.3352.
8
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Biochem Biophys Res Commun. 1973 Dec 10;55(3):881-7. doi: 10.1016/0006-291x(73)91226-6.
9
Conformations and structural transitions in polydeoxynucleotides.多脱氧核苷酸中的构象与结构转变
Biochemistry. 1975 May 6;14(9):1869-76. doi: 10.1021/bi00680a011.
10
Backbone conformational change in the A to B Transition of deoxyribonucleic acid.脱氧核糖核酸从A态到B态转变过程中的主链构象变化
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