Pohle W, Fritzsche H
Nucleic Acids Res. 1980 Jun 11;8(11):2527-35. doi: 10.1093/nar/8.11.2527.
A band at 1185 cm-1 occurs in the infrared spectra of nucleic acids in their A-type conformation which has been reproduced in the literature, too. The absorbance of this band is proportional to the fraction of the A form of DNA samples containing a mixture of different forms. The 1185 cm-1 band has been assigned tentatively to a vibration of the sugar-phosphate backbone with a fairly high contribution from the sugar moiety. Drastic dehydration of the DNA films is accompanied by a continuous intensity decrease of the 1185 cm-1 band indicating a collapse of the A form.
在核酸的 A 型构象的红外光谱中出现了一个位于 1185 cm⁻¹ 的吸收带,该吸收带在文献中也有报道。这个吸收带的吸光度与含有不同构象混合物的 DNA 样品中 A 型构象的比例成正比。1185 cm⁻¹ 处的吸收带被初步归属于糖 - 磷酸主链的振动,其中糖部分的贡献相当大。DNA 薄膜的剧烈脱水伴随着 1185 cm⁻¹ 吸收带强度的持续下降,这表明 A 型构象的崩塌。