核酸酶消化促进H1缺失染色质中的结构重排。

Nuclease digestion promotes structural rearrangements in H1-depleted chromatin.

作者信息

Weischet W O, Van Holde K E

出版信息

Nucleic Acids Res. 1980 Sep 11;8(17):3743-55. doi: 10.1093/nar/8.17.3743.

DOI:10.1093/nar/8.17.3743

PMID:7443516

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC324192/

Abstract

Digestion of H1-depleted chromatin with micrococcal nuclease at an ionic strength of 0.35M gives rise to structural rearrangements indicating nucleosomal sliding. The ionic strength necessary to reveal this effect is significantly lower than that required in the absence of an accompanying digestion. As an explanation, a model is presented in which the progressing terminal degradation of oligomeric nucleosomes is made responsible for promoting structural rearrangements.

摘要

在离子强度为0.35M的条件下，用微球菌核酸酶消化H1缺失的染色质会导致结构重排，表明核小体滑动。揭示这种效应所需的离子强度明显低于在没有伴随消化的情况下所需的离子强度。作为一种解释，提出了一个模型，其中寡聚核小体的渐进性末端降解被认为是促进结构重排的原因。