Klevan L, Crothers D M
Nucleic Acids Res. 1977 Dec;4(12):4077-89. doi: 10.1093/nar/4.12.4077.
Calf thymus chromatin, depleted in histone H1, was digested with micrococcal nuclease and fractionated by column chromatography. 140 base pair nucleosome core particles were isolated along with an unusual particle containing 2 histone octamers and 240 base pairs of DNA. Evidence is presented that the spacer DNA region is absent from these modified dinucleosomes, which appear as stable products of the digestion process. The physical properties of both particles are presented along with brief speculation on their possible origin and function.
去除组蛋白H1的小牛胸腺染色质用微球菌核酸酶消化并通过柱色谱进行分级分离。分离出了140个碱基对的核小体核心颗粒以及一种不寻常的颗粒，该颗粒含有2个组蛋白八聚体和240个碱基对的DNA。有证据表明，这些修饰的双核小体中不存在间隔DNA区域，它们似乎是消化过程的稳定产物。文中介绍了这两种颗粒的物理性质，并对其可能的起源和功能进行了简要推测。
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