Pantazis P, Longfellow D G
Prep Biochem. 1980;10(5):511-9. doi: 10.1080/00327488008061750.
A method for preparing reproducible chromatin fractions is described. Fragmentation is accomplished by expelling whole chromatin from an enclosed stainless-steel chamber through a restricted orifice utilizing low nitrogen pressure. Sheared chromatin is fractionated into two distinct heavy and light fractions by centrifugation on 5-20% linear sucrose gradient.
本文描述了一种制备可重复染色质组分的方法。通过利用低氮气压将完整的染色质从封闭的不锈钢腔室中通过一个受限的小孔排出，从而实现染色质的片段化。将剪切后的染色质在5%-20%线性蔗糖梯度上进行离心，分离成两个不同的重质和轻质组分。
