Liu G Q, Gong X C, Zhu J M, Ren G F, Fan R L, Ruan W Q
Sci Sin. 1980 Aug;23(8):1061-8.
Using cloned viruses of proven purity, and by the methods of hemagglutination inhibition, single radial hemolysis, strain-specific complement fixation and neutralization tests we have demonstrated the serological cross reaction between late H1N1 variant (Dutch/56) and H2N2 of influenza A virus with fowl and hamster antisera. Such a cross reaction is not detected with earlier H1N1 variants. Serological crossing covers variants of H2N2 virus isolated from 1957-1966 but in decreasing titers, and disappears with the last variant of H2N2 isolated late in 1967. Analysis with mono-specific antisera or antigens prepared with recombinants reveal that the hemagglutinins of late H1N1 and H2N2 are related, while their neuraminidases are distinct. We have discussed the bearing of such antigenic relationships to previous epidemiological observations on the partial protection of patients convalescent from late H1N1 disease against H2N2 and to the recombination theory for the origin of H2N2 virus.
使用经证实纯度的克隆病毒，并通过血凝抑制、单向放射溶血、菌株特异性补体结合和中和试验等方法，我们已经证明了甲型流感病毒的晚期H1N1变异株（荷兰/56）与H2N2在鸡和仓鼠抗血清中的血清学交叉反应。早期的H1N1变异株未检测到这种交叉反应。血清学交叉反应涵盖了1957年至1966年分离的H2N2病毒变异株，但效价逐渐降低，并且在1967年末分离的最后一个H2N2变异株中消失。用单特异性抗血清或重组体制备的抗原进行分析表明，晚期H1N1和H2N2的血凝素相关，而它们的神经氨酸酶不同。我们已经讨论了这种抗原关系与先前关于晚期H1N1疾病康复患者对H2N2有部分保护作用的流行病学观察以及H2N2病毒起源的重组理论之间的关联。
