Tsutsaeva A A, Ostankova L V, Drozdova O A, Gol'tsev A N
Tsitologiia. 1980 Oct;22(10):1220-5.
The growth-enhancing effect and the differentiation of colonies formed by native and frozen-thawed myelokaryocytes in semiliquid agar were observed over a period from the 2nd to the 28th day. The cultivation of bone marrow in agar was shown to form colonies consisting of haemopoietic cells as well as of stromal elements. Under the cultivation of frozen-thawed bone marrow, the retardation of proliferative activity of growing cells was observed within up to 4 days. Later on the proliferative character and the rate of the myelokaryocytes of both the types were seen identical. The question of a possible existence of haemopoietic tissue and stromal elements in the progenitor cells of normal bone marrow is being discussed.
在第2天至第28天的时间段内，观察了天然和冻融骨髓细胞在半固体琼脂中形成的集落的生长促进作用和分化情况。结果表明，在琼脂中培养骨髓可形成由造血细胞和基质成分组成的集落。在冻融骨髓的培养过程中，观察到生长细胞的增殖活性在长达4天内受到抑制。之后，两种类型的骨髓细胞的增殖特性和速率表现相同。目前正在讨论正常骨髓祖细胞中造血组织和基质成分可能存在的问题。
