Hermanová E, Viktora L, Cinátl J
Folia Biol (Praha). 1981;27(2):135-9.
The possibilities of cultivating murine bone marrow in semisolid agar and in methyl cellulose were investigated. The proliferative capacity of the haemopoietic CFU-C stem cells were demonstrated. The growth of CFU-C in fresh murine bone marrow and murine marrow short-term preserved at -75 degrees C was compared. The content of cells capable of proliferation was lower and the onset of colony formation was delayed in preserved bone marrow. Procedures appropriate for routine work were employed for evaluating haemopoietic colonies.
研究了在半固体琼脂和甲基纤维素中培养小鼠骨髓的可能性。证明了造血CFU-C干细胞的增殖能力。比较了新鲜小鼠骨髓和在-75℃短期保存的小鼠骨髓中CFU-C的生长情况。保存骨髓中能够增殖的细胞含量较低，集落形成的起始时间延迟。采用适合常规工作的方法评估造血集落。
