新鲜和冷冻小鼠骨髓的培养。——甲基纤维素的应用。
Cultivation of fresh and frozen mouse bone marrow. - Application of methyl cellulose.
作者信息
Hermanová E, Viktora L
出版信息
Folia Haematol Int Mag Klin Morphol Blutforsch. 1978;105(6):733-7.
PMID:85584
Abstract
The culture of cells of both fresh and frozen mouse bone marrow on methyl cellulose (MC) was approached. We used 1.5%-concentration of MC and proved the stem cells of fresh and frozen mouse bone marrow to proliferate and form haemopoietic colonies on MC. We established that the freezing process did not significantly decrease the proliferative capacity of CFU-C stem cells.
摘要
我们对新鲜和冷冻小鼠骨髓细胞在甲基纤维素（MC）上的培养进行了研究。我们使用了浓度为1.5%的MC，并证明新鲜和冷冻小鼠骨髓的干细胞能在MC上增殖并形成造血集落。我们确定冷冻过程不会显著降低CFU-C干细胞的增殖能力。
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