Al-Mashat R R, Taylor D J
Vet Rec. 1980 Nov 15;107(20):459-64. doi: 10.1136/vr.107.20.459.
Pure cultures of Campylobacter fetus subspecies jejuni of bovine origin were used to inoculate three ruminating calves in each of two experiments and three milk-fed calves in another. Inoculated animals all developed clinical signs which included fewer (to 41 degrees C), diarrhoea and sporadic dysentery within one to three days of inoculation. Diarrhoeic faeces were dark, mucoid, of uniform consistency and were passed for six to 15 days. The eight control animals did not develop this syndrome. C f subspecies jejuni was isolated from the faeces of all nine inoculated animals and from the faeces of two control animals in one experiment. The animals were killed 10 to 16 days following inoculation and found to have thickening of the wall of the ileum, varying degrees of inflammation of the jejunal and ileal mucosa, dark, mucoid ileal contents and enlargement of the mesenteric lymph nodes. Stunted villi, dilated crypts, some of which were filled with inflammatory cells, dilated capillaries and mononuclear cell infiltration were seen in affected small intestinal mucosa. C f subsp jejuni was isolated from the ileum, caecum and colon of all the inoculated animals and less frequently from the jejunum, gall bladder and abomasum. Antibody to the inocular strain of C f subsp jejuni was demonstrated at titres of at least 1:640 in the serum of all inoculated animals and was absent from all the control sera. The probability that C f subsp jejuni caused the syndrome produced was discussed.
采用源自牛的空肠弯曲菌胎儿亚种纯培养物，在两项实验中分别给三头反刍小牛接种，在另一项实验中给三头以奶为食的小牛接种。接种的动物均出现临床症状，包括体温降低（至41摄氏度）、腹泻和接种后1至3天内散发性痢疾。腹泻粪便呈深色、黏液状，质地均匀，持续排出6至15天。八只对照动物未出现这种综合征。在一项实验中，从所有九只接种动物的粪便以及两只对照动物的粪便中分离出空肠弯曲菌胎儿亚种。接种后10至16天处死动物，发现回肠壁增厚，空肠和回肠黏膜有不同程度的炎症，回肠内容物呈深色、黏液状，肠系膜淋巴结肿大。在受影响的小肠黏膜中可见绒毛发育不良、隐窝扩张，其中一些充满炎症细胞，毛细血管扩张和单核细胞浸润。从所有接种动物的回肠、盲肠和结肠中分离出空肠弯曲菌胎儿亚种，从空肠、胆囊和皱胃中分离出的频率较低。在所有接种动物的血清中均检测到针对空肠弯曲菌胎儿亚种接种菌株的抗体，效价至少为1:640，而所有对照血清中均未检测到。讨论了空肠弯曲菌胎儿亚种导致所产生综合征的可能性。
