Firehammer B D, Myers L L
Am J Vet Res. 1981 Jun;42(6):918-22.
Fecal samples from 127 diarrheic and 3 healthy beef calves, representing 25 herds with enteric disease, were cultured for Campylobacter, using filtration and a selective medium. Campylobacter fetus subsp jejuni (CFJ) was isolated from 51 (40%) of the diarrheic calves representing 14 (56%) herds and from the 3 clinically healthy calves (1 each in 3 of these 14 herds). Campylobacter fetus subsp intestinalis was not isolated from the calves. Fecal samples from 36 diarrheic and 20 healthy lambs representing 8 ranches were negative for Campylobacter. Isolates of CFJ from aborted lambs and from the feces of calves, ducks, dogs, and persons, appeared identical from the standpoint of morphology and physiologic reactions. In 12 calves and 5 lambs fed cultures, CFJ became colonized. Three of the calves developed diarrhea, but 2 were compromised by other disorders at the time of challenge exposure. None of the other calves or lambs developed diarrhea, but 10 of the calves and all of the lambs had frank or occult blood in the feces. Lamb intestinal loops were not distended by cultures of CFJ, but some cultures caused pathologic changes in the loops. None of 10 cultures of CFJ tested caused diarrhea in infant mice. Campylobacter fetus subsp jejuni is apparently capable of causing irritation and pathologic changes in the intestinal tract of young calves and lambs. Additional studies are needed to assess the role of CFJ in spontaneous enteric disease of calves.
从127头腹泻的和3头健康的肉牛犊中采集粪便样本，这些牛犊代表25个患有肠道疾病的牛群，使用过滤法和选择性培养基对弯曲杆菌进行培养。从51头（40%）腹泻牛犊中分离出空肠弯曲杆菌胎儿亚种（CFJ），这些腹泻牛犊代表14个牛群（56%），还从3头临床健康牛犊中分离出该菌（在这14个牛群中的3个牛群里各有1头）。未从这些牛犊中分离出肠道弯曲杆菌胎儿亚种。从36头腹泻的和20头健康的羔羊中采集粪便样本，这些羔羊代表8个牧场，样本弯曲杆菌检测呈阴性。从流产羔羊以及牛犊、鸭子、狗和人的粪便中分离出的CFJ菌株，从形态学和生理反应角度看是相同的。给12头小牛和5头羔羊投喂培养物后，CFJ定殖。其中3头小牛出现腹泻，但有2头在受到激发暴露时因其他疾病而身体虚弱。其他小牛和羔羊均未出现腹泻，但10头小牛和所有羔羊的粪便中有明显或潜血。CFJ培养物未使羔羊肠袢扩张，但有些培养物在肠袢中引起了病理变化。所检测的10份CFJ培养物均未使幼鼠出现腹泻。空肠弯曲杆菌胎儿亚种显然能够在小牛和羔羊的肠道中引起刺激和病理变化。需要进一步研究以评估CFJ在小牛自发性肠道疾病中的作用。
