Søderlund E J, Dybing E, Nordenson S, Tjelta E
Acta Pharmacol Toxicol (Copenh). 1980 Sep;47(3):175-82. doi: 10.1111/j.1600-0773.1980.tb01556.x.
The mutagenicity and azo reduction rate of N,N-diethyl-4-aminoazobenzene (DEAB) were influenced by substitution in the 4'-position with a ethyl or a fluorine group. The parent dye (DEAB) was shown to be slightly mutagenic with Salmonella typhimurium TA98 using Aroclor 1254-pretreated 9000 x g supernatant fractions from rat liver. Introduction of a 4'-ethyl group in DEAB did not affect mutagenicity of the dye, but a 4-fluoro group markedly enhanced its mutagenicity. DEAB underwent azo reduction and its reduction rate was influenced by 4'-substituents. A 4'-fluoro group in DEAB increased its azo reduction rate, while a 4'-ethyl group abolished it. Inhibitors of cytochrome P-450 also inhibited 4'-fluoro-DEAB mutagenicity and azo reduction.
N,N-二乙基-4-氨基偶氮苯（DEAB）的诱变性和偶氮还原率受4'-位上乙基或氟取代基的影响。使用来自大鼠肝脏经Aroclor 1254预处理的9000×g上清液组分，母体染料（DEAB）对鼠伤寒沙门氏菌TA98显示出轻微的诱变性。在DEAB中引入4'-乙基不影响该染料的诱变性，但4-氟基团显著增强了其诱变性。DEAB发生偶氮还原，其还原率受4'-取代基影响。DEAB中的4'-氟基团提高了其偶氮还原率，而4'-乙基基团则消除了该还原率。细胞色素P-450抑制剂也抑制4'-氟-DEAB的诱变性和偶氮还原。
