Rafii F, Hall J D, Cerniglia C E
Division of Microbiology, National Center for Toxicological Research, Food and Drug Administration, Jefferson, AR 72079, USA.
Food Chem Toxicol. 1997 Sep;35(9):897-901. doi: 10.1016/s0278-6915(97)00060-4.
Various azo dyes currently approved by the US FDA for use in foods, drugs and cosmetics are reduced by anaerobic bacteria from the human intestinal tract. These bacteria with azoreductase activities include several Clostridium species. Seven of these azo dyes and their reduction products following incubation with a Clostridium sp. were evaluated for mutagenicity in Salmonella typhimurium strains TA98 and TA100. No mutagenicity was induced in either TA98 or TA100 by any of the seven azo dyes or the reduced metabolites when tested at concentrations as high as 200 microg/plate, with or without exogenous metabolic activation by rat liver fraction S-9.
目前美国食品药品监督管理局（FDA）批准用于食品、药品和化妆品的各种偶氮染料可被来自人类肠道的厌氧细菌还原。这些具有偶氮还原酶活性的细菌包括几种梭菌属菌种。将其中七种偶氮染料及其与一种梭菌属菌种孵育后的还原产物，在鼠伤寒沙门氏菌TA98和TA100菌株中进行致突变性评估。当测试浓度高达200微克/平板时，无论有无经大鼠肝脏S-9组分进行外源性代谢活化，这七种偶氮染料及其还原代谢产物在TA98或TA100中均未诱导出致突变性。
