Kintner D, Costello D J, Levin A B, Gilboe D D
Am J Physiol. 1980 Dec;239(6):E501-9. doi: 10.1152/ajpendo.1980.239.6.E501.
In 48 separate experiments, isolated canine brain preparations were subjected to 30 min of either hypoxic (PaO2 congruent to 20 mmHg) perfusion, anoxic (PaO2 < 10 mmHg) perfusion, or total ischemia followed by reperfusion for up to 2 h with normal oxygenated blood. Unlike ischemia and anoxia, energy metabolism was sufficient during hypoxia to maintain substantial levels of ATP (48% of normal), sustain normal ion gradients, and prevent edema formation. Posthypoxia metabolism was adequate to clear accumulated lactate, enable recovery of normal tissue glucose levels, and allow return to normal levels of glycolytic intermediates. Although not as complete as that following hypoxia, recovery from cerebral edema and restoration of metabolism were better in ischemic than anoxic cortex. The reduced oxygen uptake in all groups during reoxygenation (55% of normal) indicates that all have a diminished capacity for energy metabolism. The ATP levels recovered more rapidly after 15 min of reoxygenation in the anoxic (57% of normal) than in the ischemic (21% of normal) group. Thus ATP does not appear to be directly related to recovery from edema.
在48项独立实验中，对分离的犬脑标本进行了30分钟的低氧（动脉血氧分压相当于20 mmHg）灌注、无氧（动脉血氧分压<10 mmHg）灌注或完全缺血，随后用正常氧合血再灌注长达2小时。与缺血和缺氧不同，低氧期间能量代谢足以维持较高水平的三磷酸腺苷（ATP）（为正常水平的48%），维持正常的离子梯度，并防止水肿形成。低氧后代谢足以清除积累的乳酸，使组织葡萄糖水平恢复正常，并使糖酵解中间产物恢复到正常水平。虽然不如低氧后完全，但缺血皮层脑水肿的恢复和代谢的恢复比缺氧皮层更好。复氧期间所有组的氧摄取减少（为正常水平的55%）表明所有组的能量代谢能力均降低。无氧组在复氧15分钟后三磷酸腺苷水平恢复得比缺血组更快（分别为正常水平的57%和21%）。因此，三磷酸腺苷似乎与水肿的恢复没有直接关系。
