Hillyer G V, Rivera-Marrero C
Am J Trop Med Hyg. 1982 Jul;31(4):785-9. doi: 10.4269/ajtmh.1982.31.785.
A serum was prepared by immunizing rabbits with circumoval precipitin (COP) immune complexes isolated by Protein A Sepharose affinity chromatography. This serum, positive in the COP test, was used to isolate by antibody affinity chromatography the COP antigens contained in crude schistosome egg antigens (SEA). The COP antigens were then applied to the enzyme-linked immunosorbent assay (ELISA) and compared to SEA. In a rabbit immunized repeatedly with the COP immune complexes the reactivity in the ELISA to both SEA and the COP antigens increased with time. In a primary infection of S. mansoni the reactivity to both antigen preparations rose in parallel; however, SEA showed higher reactivity. Interestingly, with the COP antigens a dramatic increase in antibody response was obtained in the serum of the 4th week in two separate groups of infected mice. This is probably a reflection of the common antigens between worms and eggs since two of the three major antibodies to the COP antigens identified previously are absorbable with lyophilized S. mansoni worms.
用经蛋白A琼脂糖亲和层析分离得到的环卵沉淀素（COP）免疫复合物免疫兔子，制备血清。该血清在COP试验中呈阳性，用于通过抗体亲和层析分离粗制血吸虫卵抗原（SEA）中含有的COP抗原。然后将COP抗原应用于酶联免疫吸附测定（ELISA），并与SEA进行比较。在一只反复用COP免疫复合物免疫的兔子中，ELISA中对SEA和COP抗原的反应性均随时间增加。在曼氏血吸虫的初次感染中，对两种抗原制剂的反应性平行上升；然而，SEA显示出更高的反应性。有趣的是，使用COP抗原时，在两组单独感染的小鼠血清中，第4周时抗体反应显著增加。这可能反映了虫体和虫卵之间的共同抗原，因为先前鉴定的针对COP抗原的三种主要抗体中的两种可被冻干的曼氏血吸虫虫体吸收。
