Dagys R, Pauliukonis A, Kazlauskas D, Mankevicius M, Simutis R
Biochem J. 1986 Aug 1;237(3):821-5. doi: 10.1042/bj2370821.
The present communication describes a novel method for estimating initial velocities (v) of enzyme-catalysed reactions. It is based on an approximation of experimental data obtained by the cubic spline function. The initial velocity of a reaction is calculated as a derivative of the approximating function at a time value equal to zero. The proposed method is usable on a computer with a FORTRAN IV program. The method can be successfully used in such cases as substantial extents of substrate conversion, the inactivation of an enzyme in the course of a reaction, the existence of large experimental error or when the reaction mechanism is unknown.
本通讯描述了一种估算酶催化反应初始速度（v）的新方法。它基于用三次样条函数获得的实验数据的近似值。反应的初始速度是通过在时间值等于零时对近似函数求导来计算的。所提出的方法可在装有FORTRAN IV程序的计算机上使用。该方法可成功应用于底物大量转化、反应过程中酶失活、存在较大实验误差或反应机制未知等情况。
