Walther D, Eulenberger K, Bier H, Delzer W, Schmidt H E, Herzmann H
Arch Exp Veterinarmed. 1980;34(4):469-81.
The antigen relationship which exists between luteinising hormone of various animal species, on the one hand, and human luteinising hormone as well as human chorionic gonadotrophin, on the other, was the background against which possible approaches were tested to the preparation of a heterologous in-vitro test for serum LH determination in cattle and sheep. The following conclusions may be drawn from the results so far obtained: --A homologous or heterologous test with specific relevance to the determination of human LH, on the basis of hLH-anti-hLH or hLH-anti-hCG is not suitable for the determination of LH in animal serum (cattle, sheep). --Quantitative and specific measurement of LH concentrations in the blood of cattle (sheep) is possible by means of an ovine-LH-anti-ovine/bovine-LH (or anti-bovine-LH) test system which is based on cross-reactivity between luteinising hormones of cattle and sheep. --Differentiated measurement of various LH concentrations in animal serum will be possible under certain specific assay conditions, with ovine LH as tracer, ovine or bovine LH as standard, and hCG antiserum. Yet, the clinical value of that heterologous radio-immuno-assay method is attributable primarily to its potential to measure peak values within the cycle to determine the time of ovulation rather than to the built-in possibility to determine base values of the cycle.
一方面，各种动物物种的促黄体生成素与另一方面的人促黄体生成素以及人绒毛膜促性腺激素之间存在的抗原关系，是测试制备用于牛和羊血清促黄体生成素测定的异源体外试验的可能方法的背景。从目前获得的结果可以得出以下结论：——基于人促黄体生成素-抗人促黄体生成素或人促黄体生成素-抗人绒毛膜促性腺激素的与人类促黄体生成素测定具有特定相关性的同源或异源试验不适用于动物血清（牛、羊）中促黄体生成素的测定。——借助基于牛和羊促黄体生成素之间交叉反应性的绵羊促黄体生成素-抗绵羊/牛促黄体生成素（或抗牛促黄体生成素）试验系统，可以对牛（羊）血液中促黄体生成素浓度进行定量和特异性测量。——在某些特定的测定条件下，以绵羊促黄体生成素为示踪剂，以绵羊或牛促黄体生成素为标准品，并用绒毛膜促性腺激素抗血清，有可能对动物血清中不同的促黄体生成素浓度进行区分测量。然而，那种异源放射免疫测定方法的临床价值主要归因于其测量周期内峰值以确定排卵时间的潜力，而不是确定周期基础值的内在可能性。
