Stupnicki R, Madej A
Endokrinologie. 1976 Oct;68(1):6-13.
A radioimmunoassay technique has been developed for measuring LH in blood plasma of cattle, sheep and pigs, by using an antiserum against bovine LH. Either ovine or bovine LH could be used for labelling and calibration curves in case of these two species, while porcine LH was to be taken in case of pigs. Working range of the calibration curve was 0.3 to 20 ng/ml. The between-assay error ranged from 17 to 7.5% between 1 to 5 ng/ml, the within-assay error from 10 to 4%, respectively. A system of quality control of the assay was developed, based on a series of pretested sera by using the chi-square function.
已开发出一种放射免疫分析技术，通过使用抗牛促黄体素抗血清来测定牛、羊和猪血浆中的促黄体素。对于牛和羊这两个物种，可用绵羊促黄体素或牛促黄体素进行标记并绘制校准曲线，而对于猪则要用猪促黄体素。校准曲线的工作范围为0.3至20纳克/毫升。在1至5纳克/毫升之间，批间误差范围为17%至7.5%，批内误差分别为10%至4%。基于一系列预先检测的血清，利用卡方函数建立了该分析的质量控制体系。
