灌注骨骼肌中的氧消耗。用衰老、新鲜和衰老-复壮红细胞进行灌注对氧消耗、组织代谢产物以及乙酰乙酸对葡萄糖利用的抑制作用。

O2 consumption, glucose metabolism and the energy status of skeletal muscle were compared in isolated rat hindquarters perfused with aged (21--35 days), fresh and aged-rejuvenated human erythrocytes. 2. The age of the erythrocytes did not affect O2 consumption, glucose utilization or lactate release either at rest or during exercise. The concentrations of ATP, phosphocreatine and lactate within the muscle were also unaffected by the use of aged erythrocytes. 3. Perfusion with acetoacetate did not inhibit glucose utilization; but, it caused a marked increase in the tissue concentration of citrate in the soleus, a slow-twitch red muscle, and a smaller increase in the gastrocnemius, which contains fast-twitch red and white fibres. Results were similar in hindquarters perfused with aged and aged-rejuvenated erythrocytes. 4. These findings suggest that perfusion with aged human erythrocytes does not cause major alterations in the metabolic performance of the isolated rat hindquarter.

比较了用衰老（21 - 35天）、新鲜和衰老恢复的人红细胞灌注的离体大鼠后肢中骨骼肌的氧气消耗、葡萄糖代谢和能量状态。2. 红细胞的年龄对静息或运动时的氧气消耗、葡萄糖利用或乳酸释放均无影响。肌肉内ATP、磷酸肌酸和乳酸的浓度也不受使用衰老红细胞的影响。3. 用乙酰乙酸灌注并不抑制葡萄糖利用；但是，它导致比目鱼肌（一种慢肌红肌）中柠檬酸的组织浓度显著增加，而在含有快肌红肌和白肌纤维的腓肠肌中增加较小。在用衰老和衰老恢复的红细胞灌注的后肢中结果相似。4. 这些发现表明，用衰老的人红细胞灌注不会引起离体大鼠后肢代谢性能的重大改变。

Oxygen consumption in perfused skeletal muscle. Effect of perfusion with aged, fresh and aged-rejuvenated erythrocytes on oxygen consumption, tissue metabolites and inhibition of glucose utilization by acetoacetate.