Rychlik W, Kupidlowska E, Nowak E, Zagórski W
Biochemistry. 1980 Nov 11;19(23):5249-55. doi: 10.1021/bi00564a015.
Wheat germ protein kinase inhibits in vitro translation of Brome Mosaic virus (BMV) RNA 1 and 2, without affecting the translation of RNA 4. Inhibition of formation of BMV polypeptides 1a and 2a is due to the arrest of initiation of polypeptide synthesis. It was found that protein kinase inhibits the formation of the 80S initiation complex with BMV RNA 1 and 2, without affecting the formation of the initiation complex with BMV RNA 4. Inhibition of protein synthesis by wheat germ protein kinase is accompanied by the phosphorylation of two ribosome-associated polypeptides, with molecular weights of 32 000 and 76 000, respectively. Both polypeptides are readily dephosphorylated by the enzyme(s) present in the cell-free extract. Their dephosphorylation is accompanied by restoration of the translational capacity of the system.
小麦胚蛋白激酶可抑制雀麦花叶病毒（BMV）RNA 1和RNA 2的体外翻译，但不影响RNA 4的翻译。BMV多肽1a和2a形成的抑制是由于多肽合成起始的阻滞。研究发现，蛋白激酶抑制与BMV RNA 1和RNA 2形成80S起始复合物，但不影响与BMV RNA 4形成起始复合物。小麦胚蛋白激酶对蛋白质合成的抑制伴随着两种核糖体相关多肽的磷酸化，其分子量分别为32000和76000。这两种多肽都很容易被无细胞提取物中的酶去磷酸化。它们的去磷酸化伴随着系统翻译能力的恢复。
