苜蓿花叶病毒RNA 4和RNA 3与核糖体-RNA起始复合物的形成。

Formation of ribosome-RNA initiation complexes with alfalfa mosaic virus RNA 4 and RNA 3.

作者信息

Pinck L, Franck A, Fritsch C

出版信息

Nucleic Acids Res. 1979 Sep 11;7(1):151-66. doi: 10.1093/nar/7.1.151.

DOI:10.1093/nar/7.1.151

PMID:114984

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC328002/

Abstract

RNA 4 of alfalfa mosaic virus (AMV) is a monocistronic messenger for the coat protein. We have determined the sequence of the 40 +/- 2 nucleotides in RNA 4 that were protected in the initiation complex formed with wheat germ 80 S ribosomes from digestion by T1 or pancreatic ribonucleases. The AUG coat protein initiation codon was near the middle of this protected region. We have found two ribosome-binding sites in RNA 3. The principal one, near the 5' end, is the initiation site for the major translation product, a 35,000 dalton protein. The second site binds ribosomes only weakly, at the beginning of the "silent" coat protein cistron, and is similar but not identical to the initiation site protection site is discussed.

摘要

苜蓿花叶病毒（AMV）的RNA 4是衣壳蛋白的单顺反子信使。我们已经确定了RNA 4中40±2个核苷酸的序列，这些核苷酸在用小麦胚芽80 S核糖体形成的起始复合物中受到保护，免受T1或胰核糖核酸酶的消化。AUG衣壳蛋白起始密码子位于该受保护区域的中间附近。我们在RNA 3中发现了两个核糖体结合位点。主要的一个位于5'端附近，是主要翻译产物35000道尔顿蛋白的起始位点。第二个位点在“沉默”衣壳蛋白顺反子开始时仅微弱地结合核糖体，并且与起始位点保护位点相似但不相同，对此进行了讨论。