Hoffman P G, Jones L A, Kuhn R W, Siiteri P K
Cancer. 1980 Dec 15;46(12 Suppl):2801-4. doi: 10.1002/1097-0142(19801215)46:12+<2801::aid-cncr2820461412>3.0.co;2-w.
An assay for cytoplasmic progesterone receptor is described in which receptor is bound to hydroxylapatite and is washed before saturation analysis. Using 3H-progesterone as ligand, the assay yields lower, more reproducible nonspecific binding than is obtained with dextran-coated charcoal techniques and may provide a clinically useful method of measuring progesterone receptor in breast carcinoma. Nuclear progesterone receptors can also be determined by the same technique.
本文描述了一种细胞质孕酮受体检测方法，该方法中受体与羟磷灰石结合，并在饱和分析前进行洗涤。以³H-孕酮作为配体，该检测方法产生的非特异性结合比葡聚糖包被活性炭技术更低、更具重复性，可能为测量乳腺癌中的孕酮受体提供一种临床有用的方法。核孕酮受体也可以通过相同技术测定。
