Hanna N, Fidler I J
Cancer Res. 1981 Feb;41(2):438-44.
Several different mouse and rat tumors were injected s.c. or i.v. into specific-pathogen-free young (3-week-old) and adult (6-week-old) nude mice. All tumors grew s.c.; however, following i.v. injections, only metastatic neoplasms produced visible pulmonary tumor foci in the 3-week-old (but not in the 6-week-old) nude mice. The quantitative differences in metastatic potential among tumor lines and clones observed in syngeneic hosts were maintained also when 3-week-old nude mice were used. The enhanced survival of tumor cells in the lungs and the large numbers of visible pulmonary metastases observed in the young nude recipients correlated closely with the low levels of natural killer cell activity detected in 3-week-old (but not in 6-week-old) nude mice. In vivo activation of natural killer cells by treatment of mice with polyinosinic-polycytidylic acid or Corynebacterium parvum, 24 hr prior to tumor challenge rendered th 3-week-old nude mice resistant to development of metastases. We conclude that specific-pathogen-free 3-week-old nude mice, which lack functional T-lymphocytes and demonstrate low natural killer cell activity, could serve as an in vivo model to ascertain the metastatic potential of allogeneic and xenogeneic tumors.
将几种不同的小鼠和大鼠肿瘤分别皮下或静脉注射到无特定病原体的幼龄（3周龄）和成年（6周龄）裸鼠体内。所有肿瘤在皮下均能生长；然而，静脉注射后，只有转移性肿瘤在3周龄（而非6周龄）裸鼠中产生可见的肺肿瘤病灶。当使用3周龄裸鼠时，在同基因宿主中观察到的肿瘤系和克隆之间转移潜能的定量差异也得以维持。在幼龄裸鼠受体中观察到的肿瘤细胞在肺中的存活率提高以及大量可见的肺转移，与在3周龄（而非6周龄）裸鼠中检测到的低水平自然杀伤细胞活性密切相关。在肿瘤攻击前24小时，用聚肌苷酸-聚胞苷酸或短小棒状杆菌处理小鼠，对自然杀伤细胞进行体内激活，可使3周龄裸鼠对转移的发生产生抗性。我们得出结论，缺乏功能性T淋巴细胞且自然杀伤细胞活性较低的无特定病原体3周龄裸鼠，可作为一种体内模型来确定同种异体和异种肿瘤的转移潜能。
