Donahoe P K, Fuller A F, Scully R E, Guy S R, Budzik G P
Ann Surg. 1981 Oct;194(4):472-80. doi: 10.1097/00000658-198110000-00010.
Mullerian inhibiting substance (MIS) was investigated for its ability to inhibit growth of a human ovarian cancer in nude mice. Biologically active preparations from newborn calf testes, obtained after sequential ion exchange chromatography, delayed or prevented growth of a human ovarian cancer (HOC-21) when 2 X 10(6) cells were preincubated with them prior to subcutaneous injection of the tumor cells into Balb/C homozygous nude mice. Preincubation of a human colon carcinoma cells (SW-48) with similar preparations of MIS failed to inhibit growth of the tumor cells in nude mice. Human serous carcinomas are thought to arise from the ovarian surface epithelium, a derivative of the coelomic epithelium of the urogenital ridge, which invaginates to form the mullerian duct early in embryonic life. The neoplastic cells of serous tumors simulate morphologically the lining cells of the fallopian tube, which are derivatives of mullerian duct epithelium. This study provides physiologic confirmation of the mullerian nature of this type of tumor and suggests that MIS may ultimately prove to be effective in its therapy.
研究了苗勒管抑制物质（MIS）抑制人卵巢癌在裸鼠体内生长的能力。从新生小牛睾丸中获得的具有生物活性的制剂，经连续离子交换色谱法处理后，当将2×10⁶个细胞在皮下注射到Balb/C纯合裸鼠体内之前与它们预孵育时，可延迟或阻止人卵巢癌（HOC - 21）的生长。用人结肠癌细胞（SW - 48）与类似的MIS制剂预孵育，未能抑制裸鼠体内肿瘤细胞的生长。人浆液性癌被认为起源于卵巢表面上皮，它是泌尿生殖嵴体腔上皮的衍生物，在胚胎早期内陷形成苗勒管。浆液性肿瘤的肿瘤细胞在形态上模拟输卵管的内衬细胞，而输卵管内衬细胞是苗勒管上皮的衍生物。本研究为这类肿瘤的苗勒管性质提供了生理学证实，并表明MIS最终可能被证明对其治疗有效。
