De Potter W P, De Smet F H
Experientia. 1980 Nov 15;36(11):1282-5. doi: 10.1007/BF01969589.
Centrifugation techniques were used to determine the subcellular distribution of noradrenaline (NA) and dopamine beta-hydroxylase (D beta H) in the rabbit iris. By application of isopycnic and differential gradient centrifugation methods 2 types of NA vesicles could be demonstrated. Of the total particle bound NA about 70% is associated with 'light' and about 30% with 'heavy' vesicles. For both types of vesicles the distribution of D beta H reflected that of NA.
采用离心技术测定去甲肾上腺素(NA)和多巴胺β-羟化酶(DβH)在兔虹膜中的亚细胞分布。通过等密度离心和差速梯度离心方法,可证明存在两种类型的NA囊泡。在与颗粒结合的总NA中,约70%与“轻”囊泡相关,约30%与“重”囊泡相关。对于这两种类型的囊泡,DβH的分布反映了NA的分布。