Timms P
Res Vet Sci. 1980 Jul;29(1):102-4.
A method based on the candle jar technique was used for the cultivation of Babesia bovis, B bigemina and B rodhaini in vitro. All three parasites multiplied within their host red blood cells and were well maintained for up to 96 h in culture. Regular changes of medium and subculture at 24 h were essential for optimal parasite survival. A 40 per cent suspension of red blood cells was found to give better results than the 10 to 12 per cent suggested by Jensen and Trager for the cultivation of Plasmodium falciparum.
一种基于烛缸技术的方法被用于在体外培养牛巴贝斯虫、双芽巴贝斯虫和罗氏巴贝斯虫。这三种寄生虫均在其宿主红细胞内增殖，并且在培养中能良好维持长达96小时。定期更换培养基以及在24小时时进行传代培养对于寄生虫的最佳存活至关重要。发现40%的红细胞悬液比Jensen和Trager建议的用于培养恶性疟原虫的10%至12%的悬液能产生更好的结果。
