Chen L T, Weiss L
Anat Embryol (Berl). 1980;159(3):277-88. doi: 10.1007/BF00317651.
The megakaryocytes in the bone marrow of steel mutant mice (S1/S1d) and their normal littermates (+/+) were studied by light and electron microscopy with special emphasis on their maturity and distribution in the hematopoietic cords. A higher percentage of megakaryocytes lying against the sinus wall, a higher percentage of the sinus perimeter covered by megakaryocytes and a higher percentage of large megakaryocytes were found in S1/S1d mice than in +/+ mice. In addition, more large megakaryocytes as well as senile megakaryocytes were observed in the spleen of S1/S1d mice than in that of +/+ mice. These observations suggest that more platelets are produced in S1/S1d mice than in +/+ mice on the basis of per unit area of the marrow tissue. Heretofore, the fate of the senile megakaryocytes in the marrow was not known. However, in S1/S1d mice senile megakaryocytes were often found entering the marrow sinuses from the hematopoietic cords. They were also seen in the lung and the spleen where degradation of senile megakaryocytes was observed. These observations suggest that senile megakaryocytes in S1/S1d mice leave the marrow and are removed by the reticuloendothelial system outside the marrow.
运用光学显微镜和电子显微镜对钢突变小鼠（S1/S1d）及其正常同窝小鼠（+/+）骨髓中的巨核细胞进行了研究，特别关注了它们在造血索中的成熟度和分布情况。与+/+小鼠相比，S1/S1d小鼠中靠在窦壁上的巨核细胞百分比更高，窦周被巨核细胞覆盖的百分比更高，以及大型巨核细胞的百分比更高。此外，与+/+小鼠相比，在S1/S1d小鼠的脾脏中观察到更多的大型巨核细胞以及衰老巨核细胞。这些观察结果表明，基于骨髓组织的单位面积，S1/S1d小鼠比+/+小鼠产生更多的血小板。在此之前，骨髓中衰老巨核细胞的命运尚不清楚。然而，在S1/S1d小鼠中，经常发现衰老巨核细胞从造血索进入骨髓窦。在肺和脾脏中也能看到它们，在那里观察到衰老巨核细胞的降解。这些观察结果表明，S1/S1d小鼠中的衰老巨核细胞离开骨髓，并被骨髓外的网状内皮系统清除。
