通过在汽巴克隆蓝F3G A-琼脂糖-聚丙烯酰胺凝胶上进行亲和电泳来测定解离常数。 - Suppr | 超能文献

The determination of dissociation constants by affinity electrophoresis on Cibacron Blue F3G A-agarose-polyacrylamide gels.

作者信息

Johnson S J, Metcalf E C, Dean P D

出版信息

Anal Biochem. 1980 Nov 15;109(1):63-6. doi: 10.1016/0003-2697(80)90010-x.

DOI:10.1016/0003-2697(80)90010-x

Abstract

摘要

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The determination of dissociation constants by affinity electrophoresis on Cibacron Blue F3G A-agarose-polyacrylamide gels.通过在汽巴克隆蓝F3G A-琼脂糖-聚丙烯酰胺凝胶上进行亲和电泳来测定解离常数。

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Electrophoretic separation of biopolymers in a matrix of polyacrylamide covalently linked to agarose.在与琼脂糖共价连接的聚丙烯酰胺基质中对生物聚合物进行电泳分离。

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Different behavior of Ferguson plot between agarose and polyacrylamide gels.琼脂糖和聚丙烯酰胺凝胶中 Ferguson 作图的不同行为。

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UV-transparent, replaceable agarose gels for molecular-sieve (capillary) electrophoresis of proteins and nucleic acids.用于蛋白质和核酸分子筛（毛细管）电泳的紫外线透明、可更换琼脂糖凝胶。

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Affinity electrophoresis: new simple and general methods of preparation of affinity gels.

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The effect of nucleotide presaturation on the chromatographic behaviour of 6-phosphogluconate dehydrogenase from Bacillus stearothermophilus on immobilized triazine dyes.核苷酸预饱和对嗜热脂肪芽孢杆菌6-磷酸葡萄糖酸脱氢酶在固定化三嗪染料上色谱行为的影响。

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The effect of ligand presaturation on the interaction of serum albumins with an immobilized Cibacron Blue 3G-A studied by affinity gel electrophoresis.通过亲和凝胶电泳研究配体预饱和对血清白蛋白与固定化汽巴蓝3G - A相互作用的影响。

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3

The genetic variants of group-specific component (vitamin D-binding protein) possess different binding characteristics for immobilized Cibacron Blue 3-GA.群体特异性成分（维生素D结合蛋白）的基因变体对固定化的汽巴蓝3-GA具有不同的结合特性。

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