Cloned neoplastic cells from a chemically-induced rat glioma: immunocytochemical characterization.

A cell clone--A15 A5--derived from an N-ethyl-N-nitrosourea (ENU)-induced glioma in a BDIX rat has been maintained in vitro for over 50 passages. These cells, which produce tumours when injected intracerebrally into syngeneic rats, possess several features of neoplastic astrocytes. In the present study A15 A5 cells were found to express both glial fibrillary acidic protein and glutamine synthetase, thus confirming their astrocytic nature.