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组织培养中实验性脑肿瘤的形态学与致瘤性研究。

Studies of the morphology and tumorigenicity of experimental brain tumours in tissue culture.

作者信息

Lantos P L, Roscoe J P, Skidmore C J

出版信息

Br J Exp Pathol. 1976 Feb;57(1):95-104.

PMID:1268044
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC2041181/
Abstract

Brain tumours induced transplacentally in BD-IX rats by a single dose of N-ethyl-N-nitrosourea have been successfully grown in tissue culture. These tumours were malignant pleomorphic gliomas. The cultured cells retained their glial morphology both in vitro and on reinjection into syngeneic hosts: astrocytes became the predominant cell type. The ability of the cells to form tumours increased with time in culture as reflected both in the decreased latency of the tumours and in the increased ability of the cells to grow in semi-solid medium.

摘要

通过单次给予N-乙基-N-亚硝基脲经胎盘诱导BD-IX大鼠产生的脑肿瘤已成功在组织培养中生长。这些肿瘤是恶性多形性胶质瘤。培养的细胞在体外以及重新注入同基因宿主后均保持其神经胶质形态：星形胶质细胞成为主要细胞类型。细胞形成肿瘤的能力随着培养时间的延长而增强，这在肿瘤潜伏期缩短以及细胞在半固体培养基中生长能力增强两方面均有体现。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ed55/2041181/0f22810fdb79/brjexppathol00139-0114-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ed55/2041181/aa88b5a95672/brjexppathol00139-0109-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ed55/2041181/09dbdee3e046/brjexppathol00139-0110-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ed55/2041181/aa11fecacf68/brjexppathol00139-0112-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ed55/2041181/823a611146b5/brjexppathol00139-0113-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ed55/2041181/0f22810fdb79/brjexppathol00139-0114-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ed55/2041181/aa88b5a95672/brjexppathol00139-0109-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ed55/2041181/09dbdee3e046/brjexppathol00139-0110-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ed55/2041181/aa11fecacf68/brjexppathol00139-0112-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ed55/2041181/823a611146b5/brjexppathol00139-0113-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ed55/2041181/0f22810fdb79/brjexppathol00139-0114-a.jpg

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引用本文的文献

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Increased ability of ethylnitrosourea-exposed brain cells to survive suspension in agar.暴露于乙基亚硝基脲的脑细胞在琼脂中悬浮时存活能力增强。
Br J Cancer. 1980 Jun;41(6):992-5. doi: 10.1038/bjc.1980.180.
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本文引用的文献

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Virology. 1964 Jun;23:291-4. doi: 10.1016/0042-6822(64)90301-0.
2
Teratogenic and carcinogenic effects in the offspring after single injection of ethylnitrosourea to pregnant rats.对怀孕大鼠单次注射乙基亚硝基脲后子代的致畸和致癌作用。
Nature. 1966 Jun 25;210(5043):1378-9. doi: 10.1038/2101378a0.
3
Morphological studies of rat brain tumors induced by N-nitrosomethylurea.N-亚硝基甲基脲诱导的大鼠脑肿瘤的形态学研究。
Experientia. 1980 Feb 15;36(2):194-6. doi: 10.1007/BF01953727.
4
Alginate: a reversible semi-solid medium for investigating cell transformation.藻酸盐：一种用于研究细胞转化的可逆半固体培养基。
Br J Cancer. 1982 Dec;46(6):965-9. doi: 10.1038/bjc.1982.309.
5
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Br J Cancer. 1984 Mar;49(3):357-61. doi: 10.1038/bjc.1984.56.
6
The effect of dexamethasone on vascular permeability of experimental brain tumours.地塞米松对实验性脑肿瘤血管通透性的影响。
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7
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8
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9
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Br J Cancer. 1978 Mar;37(3):424-33. doi: 10.1038/bjc.1978.63.
J Neurosurg. 1971 Mar;34(3):335-40. doi: 10.3171/jns.1971.34.3.0335.
4
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5
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8
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