Etienne J, Grüber A, Polonovski J
Biochim Biophys Acta. 1980 Sep 8;619(3):693-8. doi: 10.1016/0005-2760(80)90119-8.
In the rat, blood coagulation is accompanied by activation of a plasma phospholipase A2 due to a factor contained in blood platelets and released in plasma by platelet lysis. This activity can be obtained in vitro by treating plasma devoid of platelets with a platelet lysate. The results reported in this communication show the activity of serum phospholipase to result from an association of two proteins, inactive when isolated, one originating from plasma, the other from platelets, which can be broken down by fractionation on a column of Blue Sepharose CL-6B.
在大鼠中，血液凝固伴随着血浆磷脂酶A2的激活，这是由于血小板中所含的一种因子，通过血小板裂解而释放到血浆中。这种活性可以通过用血小板裂解物处理不含血小板的血浆在体外获得。本通讯中报道的结果表明，血清磷脂酶的活性是由两种蛋白质结合产生的，这两种蛋白质单独分离时无活性，一种来自血浆，另一种来自血小板，它们可以通过在蓝色琼脂糖凝胶CL-6B柱上进行分级分离而被分解。
