Departments of Microbiology and Cell Science and Agricultural Engineering, University of Florida, Gainesville, Florida 32611.
Appl Environ Microbiol. 1984 Jul;48(1):127-36. doi: 10.1128/aem.48.1.127-136.1984.
Ultrastructural examinations were performed on biofilms from eight anaerobic fixed-bed reactors filled with various packing materials and operated on fresh swine waste. By using light, UV, scanning, and transmission electron microscopy, the distribution of a diverse microbial population composed of bacteria and a few yeasts was determined. This is the first time that the ultrastructure of in situ anaerobic digestor biofilms has been reported. A large number of methanogenic bacteria were identified by their fluorescence under 420 nm of radiation. Of these, two morphologically distinct types were most prevalent in the films. Methanothrix spp. was present in high numbers at the film surface, whereas Methanosarcina spp. were commonly embedded in the lower regions of the film. Inhabitants of the film were surrounded by an exopolysaccharide matrix that was very dense toward the base. An extensive network of channels was observed throughout the matrix that may facilitate gas and nutrient exchange to the lower regions of the film.
对填充有各种包装材料并在新鲜猪粪上运行的八个厌氧固定床反应器中的生物膜进行了超微结构检查。通过使用光、紫外线、扫描和透射电子显微镜，确定了由细菌和一些酵母组成的多样化微生物种群的分布。这是首次报道原位厌氧消化器生物膜的超微结构。通过 420nm 辐射下的荧光，大量产甲烷菌被鉴定出来。其中，两种形态明显不同的类型在薄膜中最为普遍。在薄膜表面存在大量的 Methanothrix spp.，而 Methanosarcina spp. 则通常嵌入在薄膜的下部区域。薄膜中的生物被包裹在非常致密的胞外多糖基质中。在整个基质中观察到广泛的通道网络，这可能有利于气体和营养物质向薄膜的下部区域交换。
