Cerone-McLernon A M, Furness G
Can J Microbiol. 1980 Sep;26(9):1147-52. doi: 10.1139/m80-189.
DNA extracted by a standard method from Mycoplasma hominis Sprott, resistant to 100 micrograms tetracycline, permitted the quantitative genetic transformation of tetracycline-sensitive Mycoplasma salivarium to resistance. The yield was 1 microgram DNA/10(9) cells. This DNA enabled determination of the optimum conditions for making M. Salivarium competent with CaCl2 and for studying some factors affecting transformation. Mycoplasma salivarium was transformed to resistance to 10, 20, and 30 micrograms tetracycline but not to 40 micrograms. The optimum DNA concentration for transforming resistance to 10, 20, and 30 micrograms tetracycline was the same, i.e., 50 micrograms DNA/10(8) viable cells. Treatment with DNase indicated that DNA uptake took 30 min. Competition between transforming DNA and DNA from calf thymus and M. salivarium tets inhibited transformation.
采用标准方法从对100微克四环素耐药的人型支原体斯普罗特菌株中提取的DNA，可使对四环素敏感的唾液支原体定量遗传转化为耐药菌株。产量为1微克DNA/10⁹个细胞。这种DNA能够确定用氯化钙使唾液支原体感受态的最佳条件以及研究影响转化的一些因素。唾液支原体被转化为对10、20和30微克四环素耐药，但对40微克四环素不耐药。将其转化为对10、20和30微克四环素耐药的最佳DNA浓度相同，即50微克DNA/10⁸个活细胞。用DNA酶处理表明DNA摄取需30分钟。转化DNA与来自小牛胸腺和唾液支原体的DNA之间的竞争抑制了转化。
