Dollbaum C M, Anderson L W, Smith H S
Cancer Lett. 1980 Dec;11(2):121-7. doi: 10.1016/0304-3835(80)90102-0.
We have developed an epithelial cell line derived from mouse liver which represents a spectrum of malignant progression. When inoculated into mice at low passage, the cells induced benign cysts; after extensive subcultures, the same line induced low grade adenocarcinomas. A variant of these cells with increased invasive and metastatic potential was selected. In culture, growth in methocel correlated with acquisition of malignant potential while increased homotypic aggregation correlated with metastatic ability. These cultures should be extremely valuable for studies on the nature of epithelial cell malignancy, the most common form of human cancer.
我们已经开发出一种源自小鼠肝脏的上皮细胞系，它代表了一系列恶性进展过程。当以低传代次数接种到小鼠体内时，这些细胞会诱导形成良性囊肿；经过广泛传代培养后，同一细胞系会诱导形成低级别腺癌。我们筛选出了这些细胞的一种具有更高侵袭和转移潜能的变体。在培养过程中，在甲基纤维素中生长与获得恶性潜能相关，而增加的同型聚集与转移能力相关。这些培养物对于研究上皮细胞恶性肿瘤（人类癌症最常见的形式）的本质应该具有极高的价值。
