Anderson L W, Smith H S
Br J Exp Pathol. 1979 Dec;60(6):575-81.
An epithelial cell line, NMuLi, derived from normal mouse liver, produced a variety of tumours when inoculated s.c. into newborn mice. The inoculation of early-passage cells resulted in cystadenomas and proliferating cysts, while adenocarcinomas and carcinosarcomas were produced from cells that had been extensively subcultured. To determine the mechanism involved in the progression to malignancy, single-cell clones were isolated from early- and late-passage NMuLi cells. When inoculated into mice, early-passage clones were similar to the original population in that they initially formed benign cysts but produced increasingly aberrant tumours after extensive subculture. Inoculation of the late-passage clones resulted in only malignant tumours. These observations suggest that passage in culture induced an initially benign epithelial cell population to progress toward malignancy; thus early passage NMuLi cells represent a good in vitro model of the premalignant state.
一种源自正常小鼠肝脏的上皮细胞系NMuLi，经皮下接种到新生小鼠体内后会产生多种肿瘤。接种早期传代细胞会导致囊腺瘤和增殖性囊肿，而腺癌和癌肉瘤则由经过大量传代培养的细胞产生。为了确定向恶性肿瘤进展所涉及的机制，从早期和晚期传代的NMuLi细胞中分离出单细胞克隆。当接种到小鼠体内时，早期传代克隆与原始细胞群体相似，即它们最初形成良性囊肿，但在大量传代培养后会产生越来越异常的肿瘤。接种晚期传代克隆只会导致恶性肿瘤。这些观察结果表明，在培养中传代会促使最初良性的上皮细胞群体向恶性肿瘤进展；因此，早期传代的NMuLi细胞代表了一种良好的癌前状态体外模型。
