Stanley J, Rottier P, Davies J W, Zabel P, Van Kammen A
Nucleic Acids Res. 1978 Dec;5(12):4505-22. doi: 10.1093/nar/5.12.4505.
Evidence is presented for the presence of a protein covalently bound to the 5' termini of both M and B RNA components of CPMV. The protein is found to be linked in both cases to the 5' phosphate of the dinucleotide pUpAp, derived by ribonuclease digestion of the RNA. The intact protein is not required for infectivity or for in vitro translation of the RNA in cell-free extracts.
有证据表明,在豇豆花叶病毒(CPMV)的M和B RNA组分的5'末端存在一种与之共价结合的蛋白质。在这两种情况下,都发现该蛋白质与通过RNA核糖核酸酶消化得到的二核苷酸pUpAp的5'磷酸基团相连。完整的蛋白质对于感染性或无细胞提取物中RNA的体外翻译不是必需的。