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一种与豇豆花叶病毒基因组的两个RNA组分的5'末端相连的蛋白质。

A protein linked to the 5' termini of both RNA components of the cowpea mosaic virus genome.

作者信息

Stanley J, Rottier P, Davies J W, Zabel P, Van Kammen A

出版信息

Nucleic Acids Res. 1978 Dec;5(12):4505-22. doi: 10.1093/nar/5.12.4505.

DOI:10.1093/nar/5.12.4505
PMID:745988
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC342769/
Abstract

Evidence is presented for the presence of a protein covalently bound to the 5' termini of both M and B RNA components of CPMV. The protein is found to be linked in both cases to the 5' phosphate of the dinucleotide pUpAp, derived by ribonuclease digestion of the RNA. The intact protein is not required for infectivity or for in vitro translation of the RNA in cell-free extracts.

摘要

有证据表明，在豇豆花叶病毒（CPMV）的M和B RNA组分的5'末端存在一种与之共价结合的蛋白质。在这两种情况下，都发现该蛋白质与通过RNA核糖核酸酶消化得到的二核苷酸pUpAp的5'磷酸基团相连。完整的蛋白质对于感染性或无细胞提取物中RNA的体外翻译不是必需的。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a92a/342769/02655b927ab7/nar00473-0051-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a92a/342769/8bc6d73b7880/nar00473-0045-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a92a/342769/3b7ab91ec266/nar00473-0046-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a92a/342769/6c83259c9e22/nar00473-0047-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a92a/342769/5bc4d4063b97/nar00473-0048-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a92a/342769/02655b927ab7/nar00473-0051-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a92a/342769/8bc6d73b7880/nar00473-0045-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a92a/342769/3b7ab91ec266/nar00473-0046-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a92a/342769/6c83259c9e22/nar00473-0047-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a92a/342769/5bc4d4063b97/nar00473-0048-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a92a/342769/02655b927ab7/nar00473-0051-a.jpg

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A protein linked to the 5' termini of both RNA components of the cowpea mosaic virus genome.一种与豇豆花叶病毒基因组的两个RNA组分的5'末端相连的蛋白质。
Nucleic Acids Res. 1978 Dec;5(12):4505-22. doi: 10.1093/nar/5.12.4505.
2
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J Gen Virol. 1992 Nov;73 ( Pt 11):2775-84. doi: 10.1099/0022-1317-73-11-2775.

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本文引用的文献

1
The relationship between the components of cowpea mosaic virus. I. Two ribonucleoprotein particles necessary for the infectivity of CPMV.豇豆花叶病毒各组分之间的关系。I. 豇豆花叶病毒感染性所需的两种核糖核蛋白颗粒。
Virology. 1968 Feb;34(2):312-8. doi: 10.1016/0042-6822(68)90241-9.
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A two-dimensional fractionation procedure for radioactive nucleotides.一种用于放射性核苷酸的二维分级分离程序。
J Mol Biol. 1965 Sep;13(2):373-98. doi: 10.1016/s0022-2836(65)80104-8.
3
Suppression of RNA precipitation during Cs2SO4 density gradient centrifugation.
小麦胚芽和豇豆胚中存在的一种抑制活性对植物病毒多聚蛋白切割的抑制作用。
J Virol. 1987 Mar;61(3):912-5. doi: 10.1128/JVI.61.3.912-915.1987.
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Evidence That the 32,000-Dalton Protein Encoded by Bottom-Component RNA of Cowpea Mosaic Virus is a Proteolytic Processing Enzyme.豇豆花叶病毒底部组分RNA编码的32000道尔顿蛋白质是一种蛋白水解加工酶的证据。
J Virol. 1984 Apr;50(1):183-90. doi: 10.1128/JVI.50.1.183-190.1984.
5
Orientation of the cleavage map of the 200-kilodalton polypeptide encoded by the bottom-component RNA of cowpea mosaic virus.豇豆花叶病毒底部组分RNA编码的200千道尔顿多肽切割图谱的定位
J Virol. 1983 May;46(2):614-9. doi: 10.1128/JVI.46.2.614-619.1983.
6
Expression of the Bottom-Component RNA of Cowpea Mosaic Virus: Evidence that the 60-Kilodalton VPg Precursor Is Cleaved into Single VPg and a 58-Kilodalton Polypeptide.豇豆花叶病毒底部组分RNA的表达：60千道尔顿VPg前体被切割成单个VPg和一个58千道尔顿多肽的证据
J Virol. 1982 May;42(2):630-5. doi: 10.1128/JVI.42.2.630-635.1982.
7
Antibodies Against the Genome-Linked Protein VPg of Cowpea Mosaic Virus Recognize a 60,000-Dalton Precursor Polypeptide.抗豇豆花叶病毒基因组连接蛋白VPg的抗体识别一种60,000道尔顿的前体多肽。
J Virol. 1982 Mar;41(3):1083-8. doi: 10.1128/JVI.41.3.1083-1088.1982.
8
Expression of Middle-Component RNA of Cowpea Mosaic Virus: In Vitro Generation of a Precursor to Both Capsid Proteins by a Bottom-Component RNA-Encoded Protease from Infected Cells.豇豆花叶病毒中间组分RNA的表达：感染细胞中由底部组分RNA编码的蛋白酶体外产生两种衣壳蛋白的前体
J Virol. 1982 Jan;41(1):8-17. doi: 10.1128/JVI.41.1.8-17.1982.
9
Expression of bottom component RNA of cowpea mosaic virus in cowpea protoplasts.豇豆花叶病毒底部组分RNA在豇豆原生质体中的表达
J Virol. 1980 Nov;36(2):366-73. doi: 10.1128/JVI.36.2.366-373.1980.
10
In vitro expression of a full-length DNA copy of cowpea mosaic virus B RNA: identification of the B RNA encoded 24-kd protein as a viral protease.体外表达豇豆花叶病毒 B 株 RNA 的全长 DNA 拷贝：鉴定 B RNA 编码的 24kD 蛋白为病毒蛋白酶。
EMBO J. 1987 Mar;6(3):549-54. doi: 10.1002/j.1460-2075.1987.tb04789.x.
在硫酸铯密度梯度离心过程中对RNA沉淀的抑制
Biochem Biophys Res Commun. 1966 Jun 13;23(5):612-8. doi: 10.1016/0006-291x(66)90443-8.
4
Ribonucleic acid isolated by cesium chloride centrifugation.通过氯化铯离心法分离得到的核糖核酸。
Biochemistry. 1974 Jun 4;13(12):2633-7. doi: 10.1021/bi00709a025.
5
Polyadenylate sequences in the ribonucleic acids of cowpea mosaic virus.豇豆花叶病毒核糖核酸中的聚腺苷酸序列。
Virology. 1973 Nov;56(1):198-206. doi: 10.1016/0042-6822(73)90299-7.
6
The labelling of proteins to high specific radioactivities by conjugation to a 125I-containing acylating agent.通过与含¹²⁵I的酰化剂结合将蛋白质标记至高比放射性。
Biochem J. 1973 Jul;133(3):529-39. doi: 10.1042/bj1330529.
7
Plant viruses with a multipartite genome.具有多分体基因组的植物病毒。
Adv Virus Res. 1974;19:37-149. doi: 10.1016/s0065-3527(08)60659-4.
8
Gel electrophoresis of RNA under denaturing conditions.变性条件下RNA的凝胶电泳。
Biochim Biophys Acta. 1973 Oct 26;324(3):320-33. doi: 10.1016/0005-2787(73)90278-5.
9
Genetic analysis of cowpea mosaic virus mutants by supplementation and reassortment tests.通过互补和重配测试对豇豆花叶病毒突变体进行遗传分析。
Virology. 1976 Mar;70(1):151-63. doi: 10.1016/0042-6822(76)90245-2.
10
Cowpea mosaic virus RNAs have neither m7GpppN ... nor mono-, di- or triphosphates at their 5' ends.豇豆花叶病毒RNA在其5'末端既没有m7GpppN……也没有一磷酸、二磷酸或三磷酸。
Cell. 1977 May;11(1):73-82. doi: 10.1016/0092-8674(77)90318-x.