咖啡因抑制指数生长期的中国仓鼠细胞中7-溴甲基苯并(a)蒽-DNA加合物的切除，但对静止期细胞无此作用。
Caffeine inhibits excision of 7-bromomethylbenz (a) anthracene-DNA adducts from exponentially growing but not from stationary phase Chinese hamster cells.
作者信息
Friedlos F, Roberts J J
出版信息
Nucleic Acids Res. 1978 Dec;5(12):4795-803. doi: 10.1093/nar/5.12.4795.
Abstract
Excision of 7-bromomethylbenz(a)anthracene (7-BMBA)-DNA adducts from exponentially growing cultures of Chinese hamster V79-379A cells followed logarithmic kinetics with a half of approximately 20 hrs. Post-treatment incubation in the presence of a sub-toxic concentration of caffeine markedly reduced this loss. Caffeine brought about a concomitant increase in overall DNA synthetic rate in treated exponential cultures. Excision in stationary, non-DNA-replicating cultures, was slower and caffeine did not affect this reduced rate of excision. These findings lend support to a previous proposition that the caffeine-induced inhibition of elongation of nascent DNA on a template containing chemical lesions results in an interference with the excision repair mechanism that removes these lesions.
摘要
从指数生长的中国仓鼠V79 - 379A细胞培养物中切除7 - 溴甲基苯并(a)蒽（7 - BMBA）- DNA加合物遵循对数动力学，半衰期约为20小时。在亚毒性浓度的咖啡因存在下进行处理后的孵育显著减少了这种损失。咖啡因使处理后的指数生长培养物中的总体DNA合成速率同时增加。在静止的、不进行DNA复制的培养物中，切除速度较慢，咖啡因不影响这种降低的切除速率。这些发现支持了先前的一个观点，即咖啡因诱导的对含有化学损伤模板上新生DNA延伸的抑制会干扰去除这些损伤的切除修复机制。
相似文献
1
Caffeine inhibits excision of 7-bromomethylbenz (a) anthracene-DNA adducts from exponentially growing but not from stationary phase Chinese hamster cells.咖啡因抑制指数生长期的中国仓鼠细胞中7-溴甲基苯并(a)蒽-DNA加合物的切除，但对静止期细胞无此作用。
Nucleic Acids Res. 1978 Dec;5(12):4795-803. doi: 10.1093/nar/5.12.4795.
2
Caffeine-inhibited DNA repair in 7-bromomethylbenz (a)-anthracene-treated Chinease hamster cells: formation of breaks in parental DNA and inhibition of ligation of nascent DNA as a mechanism for enhancement of lethality and chromosome damage.咖啡因对经7-溴甲基苯并(a)蒽处理的中国仓鼠细胞的DNA修复有抑制作用：亲本DNA断裂的形成以及新生DNA连接的抑制作为致死率增加和染色体损伤增强的一种机制。
Mutat Res. 1978 May;50(2):263-75. doi: 10.1016/0027-5107(78)90030-1.
3
Effects of cis-platinum(II) diamminedichloride on survival and the rate of DNA synthesis in synchronously growing Chinese hamster V79-379A cells in the absence and presence of caffeine inhibited post-replication repair; evidence for an inducible repair mechanism.
Chem Biol Interact. 1978 Oct;23(1):99-110. doi: 10.1016/0009-2797(78)90044-3.
4
Inhibition by caffeine of post-replication repair in Chinese hamster cells treated with 7-bromomethylbenz(a)anthracene: enhancement of toxicity, chromosome damage and inhibition of ligation of newly-synthesized DNA.咖啡因对经7-溴甲基苯并(a)蒽处理的中国仓鼠细胞复制后修复的抑制作用：毒性增强、染色体损伤及新合成DNA连接抑制。
Chem Biol Interact. 1977 Jun;17(3):265-90. doi: 10.1016/0009-2797(77)90091-6.
5
Excision of 7-bromomethylbenz[a]anthracene--DNA adducts in replicating mammalian cells.
Biochemistry. 1977 Apr 5;16(7):1499-503. doi: 10.1021/bi00626a039.
6
Inhibition by caffeine of post-replication repair in Chinese hamster cells treated with cis platinum (II) Diamminedichloride: the extent of platinum binding to template DNA in relation to the size of low molecular weight nascent DNA.
Chem Biol Interact. 1976 Mar;12(3-4):375-90. doi: 10.1016/0009-2797(76)90052-1.
7
Post-replication repair of DNA in Chinese hamster cells treated with cis platinum (II) diamine dichloride. Enhancement of toxicity and chromosome damage by caffeine.
Mutat Res. 1975 Dec;33(2-3):279-84. doi: 10.1016/0027-5107(75)90203-1.
8
[Postreplicative DNA repair in UV-sensitive Chinese hamster cell clones].[紫外线敏感的中国仓鼠细胞克隆中的复制后DNA修复]
Tsitologiia. 1980 Sep;22(9):1085-95.
9
10
[Defect in the caffeine-dependent postreplication DNA repair in UV-sensitive Chinese hamster clone cells].[对紫外线敏感的中国仓鼠克隆细胞中咖啡因依赖性复制后DNA修复缺陷]
Tsitologiia. 1984 Nov;26(11):1295-301.
引用本文的文献
1
Regulation of DNA repair in serum-stimulated xeroderma pigmentosum cells.血清刺激的着色性干皮病细胞中DNA修复的调控
J Cell Biol. 1984 Oct;99(4 Pt 1):1275-81. doi: 10.1083/jcb.99.4.1275.
本文引用的文献
1
Structure and activity in chemical carcinogenesis: reactivity and carcinogenicity of 7-bromomethylbenz[alpha]anthracene and 7-bromomethyl-12-methylbenz[alpha]anthracene.
Eur J Cancer (1965). 1970 Oct;6(5):417-23. doi: 10.1016/0014-2964(70)90040-x.
2
Mutagenicity to mammalian cells of epoxides and other derivatives of polycyclic hydrocarbons.多环烃的环氧化物及其他衍生物对哺乳动物细胞的致突变性。
Proc Natl Acad Sci U S A. 1971 Dec;68(12):3195-9. doi: 10.1073/pnas.68.12.3195.
3
Quantitative aspects of the repair of alkylated DNA in cultured mammalian cells. I. The effect on HeLa and Chinese hamster cell survival of alkylation of cellular macromolecules.培养的哺乳动物细胞中烷基化DNA修复的定量研究。I. 细胞大分子烷基化对HeLa细胞和中国仓鼠细胞存活的影响。
Chem Biol Interact. 1971 Feb;3(1):29-47. doi: 10.1016/0009-2797(71)90024-x.
4
Reaction of 7-bromomethylbenz(a)anthracene with nucleic acids, polynucleotides, and nucleosides.7-溴甲基苯并(a)蒽与核酸、多核苷酸和核苷的反应。
Biochemistry. 1971 Nov;10(23):4323-30. doi: 10.1021/bi00799a026.
5
Inhibition of post-replication repair of alkylated DNA by caffeine in Chinese hamster cells but not HeLa cells.
Chem Biol Interact. 1973 Oct;7(4):241-64. doi: 10.1016/0009-2797(73)90027-6.
6
7
Carcinogenic activity of some benz(a)anthracene derivatives in newborn mice.某些苯并(a)蒽衍生物对新生小鼠的致癌活性。
Br J Cancer. 1972 Dec;26(6):461-5. doi: 10.1038/bjc.1972.63.
8
Removal of bound carcinogen during DNA repair in nondividing human lymphocytes.非分裂期人类淋巴细胞DNA修复过程中结合致癌物的去除
Cancer Res. 1972 Sep;32(9):1855-60.
9
Inhibition of DNA repair replication by DNA binding drugs which sensitize cells to alkylating agents and X-rays.
Proc Soc Exp Biol Med. 1972 Nov;141(2):543-7. doi: 10.3181/00379727-141-36818.
10
Effects of coumarin, pyronin Y, 6,9-dimethyl 2-methylthiopurine and caffeine on excision repair and recombination repair in Escherichia coli.香豆素、派洛宁Y、6,9-二甲基-2-甲基硫代嘌呤及咖啡因对大肠杆菌切除修复和重组修复的影响
J Gen Microbiol. 1972 Apr;70(2):221-30. doi: 10.1099/00221287-70-2-221.
Zotero 插件