Massey E D, Butler W H
Chem Biol Interact. 1981 Feb;34(1):31-8. doi: 10.1016/0009-2797(81)90087-9.
Alterations in the liver of rats 6 h after a dose of phenobarbitone have been studied by subcellular fractionation, conventional electron microscopy and morphometric analysis. The area immediately surrounding the central vein was the only area to undergo any alterations. There was a morphometrically measurable but not observable cellular hypertrophy of 71% whilst the hepatocyte complement of rough endoplasmic reticulum (RER) and smooth endoplasmic reticulum (SER) was increased by 72% and 93% respectively. The increases in RER and SER were not apparent by observation and it is assumed that they have been diluted by the cell hypertrophy to 1% and 22% which must be below the threshold for detection by subjective observation. Following subcellular fractionation and measurement of microsomal protein, there was no significant difference in the level of microsomes isolated from control or treated rats. Therefore, the morphometrically measured increase in RER and SER would appear to be restricted to a relatively small population of hepatocytes adjacent to the central vein. Such an increase would represent only a small percentage of total microsomes in a homogenate and would almost certainly be masked by variation in animals and techniques. Disruption of RER was also observed in hepatocytes that would proliferate their SER should phenobarbitone treatment have been continued. Therefore this RER disruption would seem in no way to interfere with the process of membrane and enzyme synthesis.
通过亚细胞分级分离、传统电子显微镜检查和形态计量分析，研究了给予苯巴比妥剂量6小时后大鼠肝脏的变化。中央静脉周围的区域是唯一发生任何变化的区域。存在形态计量学上可测量但肉眼不可见的71%的细胞肥大，同时粗面内质网（RER）和滑面内质网（SER）的肝细胞含量分别增加了72%和93%。通过观察，RER和SER的增加并不明显，据推测，它们已被细胞肥大稀释至1%和22%，这肯定低于主观观察的检测阈值。在进行亚细胞分级分离并测量微粒体蛋白后，从对照大鼠或处理大鼠分离的微粒体水平没有显著差异。因此，形态计量学测量的RER和SER的增加似乎仅限于中央静脉附近相对较少的肝细胞群体。这种增加仅占匀浆中总微粒体的一小部分，几乎肯定会被动物和技术的差异所掩盖。如果继续给予苯巴比妥治疗，在SER会增殖的肝细胞中也观察到了RER的破坏。因此，这种RER破坏似乎绝不会干扰膜和酶的合成过程。
