Stocker C, Martínez Peralta L, Kratzberg T, Lohmann F, Bruns M
Heinrich-Pette-Institut für Experimentelle Virologie und Immunologie, Universität Hamburg, Germany.
J Gen Virol. 1994 Dec;75 ( Pt 12):3431-9. doi: 10.1099/0022-1317-75-12-3431.
Continuous cultivation of murine L cells infected with lymphocytic choriomeningitis virus strain Armstrong leads to production of L(Arm) cells, which produce a predominantly cell-associated attenuated variant, the L(Arm) virus. The relatively few infectious particles that are released have lost the ability to form plaques on L cells and to cause illness in mice even if inoculated intracerebrally. Based on equal protein M(r)s, antigenicity and protein kinase activity, essentially identical results were obtained for the purified Armstrong and L(Arm) viruses. There was also no difference in production and release of particles with the potential to cause homologous interference. Such particles consisted of two types, one of which was highly susceptible to u.v.-irradiation, the other was highly resistant. In the case of the L(Arm) virus interfering particles, it appears that the u.v.-irradiation-susceptible forms represented infectious virus. Purified L(Arm) virus particles contained considerable quantities of subgenomic forms of (small) S- and (large) L-RNA and their complementary counterparts, which all appeared to be replicated autonomously in an unenriched manner.
持续培养感染淋巴细胞性脉络丛脑膜炎病毒阿姆斯特朗株的小鼠L细胞，会产生L(Arm)细胞，该细胞产生一种主要与细胞相关的减毒株L(Arm)病毒。释放出的相对较少的感染性颗粒已失去在L细胞上形成噬斑的能力，即使脑内接种也无法使小鼠致病。基于相同的蛋白质相对分子质量、抗原性和蛋白激酶活性，纯化后的阿姆斯特朗病毒和L(Arm)病毒得到了基本相同的结果。在产生和释放具有同源干扰潜力的颗粒方面也没有差异。此类颗粒有两种类型，其中一种对紫外线照射高度敏感，另一种则高度耐受。就L(Arm)病毒干扰颗粒而言，似乎对紫外线照射敏感的形式代表感染性病毒。纯化后的L(Arm)病毒颗粒含有大量亚基因组形式的（小）S-RNA和（大）L-RNA及其互补对应物，它们似乎都以未富集的方式自主复制。
