从小牛胸腺中纯化并部分鉴定一种DNA聚合酶α。
Purification and partial characterization of a DNA polymerase alpha species from calf thymus.
作者信息
Grosse F, Krauss G
出版信息
Nucleic Acids Res. 1980 Dec 11;8(23):5703-14. doi: 10.1093/nar/8.23.5703.
Abstract
We have purified a DNA polymerase alpha species from calf thymus to near homogeneity. The enzyme sediments at 5.7 S and contains two polypeptides of 123000 and 134000 daltons in about equimolar ratio. The enzyme is inhibited by aphidicolin and N-ethylmaleimide, and retains its activity in buffers containing moderate salt conditions. Activated DNA is a better substrate than poly-(dA) . (dT) 10.
摘要
我们已将小牛胸腺中的一种DNA聚合酶α纯化至接近均一状态。该酶在5.7 S处沉降，含有两条分子量分别为123000和134000道尔顿的多肽，二者比例约为等摩尔。该酶受阿非迪霉素和N-乙基马来酰亚胺抑制，在含有中等盐浓度的缓冲液中可保持其活性。活化的DNA是比聚（dA）·（dT）10更好的底物。
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