Petrov A I
Nucleic Acids Res. 1980 Dec 11;8(23):5913-29. doi: 10.1093/nar/8.23.5913.
Complex formation between poly (U) and pyrimidine nucleosides, uridine and cytidine, was observed using spin labeling technique. The binding of these nucleosides with poly (U) takes place within a narrow range of their concentration and is characterized by a relatively strong cooperativity. It is shown, that both hydrogen bonding and stacking interaction contribute to the complex stability. Some thermodynamic parameters of the process were obtained from the binding isotherms. At 21 degrees C the equilibrium constants for nucleation were found to be 0.23 M-1 and 0.42 M-1, and those for chain growth 2.63 M-1 and 2.19 M-1 for uridine and cytidine respectively. Complex formation of poly (U) with adenosine was also studied by spin labeling method.
利用自旋标记技术观察到聚（U）与嘧啶核苷、尿苷和胞苷之间的复合物形成。这些核苷与聚（U）的结合发生在其浓度的狭窄范围内，其特征是具有相对较强的协同性。结果表明，氢键和堆积相互作用都有助于复合物的稳定性。该过程的一些热力学参数是从结合等温线获得的。在21摄氏度时，发现尿苷和成核的平衡常数分别为0.23 M-1和0.42 M-1，链增长的平衡常数分别为2.63 M-1和2.19 M-1，胞苷的情况类似。还通过自旋标记法研究了聚（U）与腺苷的复合物形成。
