嘧啶核苷和胞嘧啶的堆积自缔合：甲基化和硫醇化的影响。
Stacking self-association of pyrimidine nucleosides and of cytosines: effects of methylation and thiolation.
作者信息
Plesiewicz E, Stepień E, Bolewska K, Wierzchowski K L
出版信息
Nucleic Acids Res. 1976 May;3(5):1295-306. doi: 10.1093/nar/3.5.1295.
Abstract
Stacking self-association equilibria in aqueous solutions of m3uridine, m34,2',3',5'uridine, 2'-deoxyuridine, m13,4,4cytosine, m14,4,4,5cytosine, s2cytidine and s4thymidine were studied at various temperatures by vapour-pressure osmometry. Equilibrium constants Kst's were computed on the assumption of the isodesmic model of self-association. Enthalpies of association were also obtained from the temperature dependence of Kst according to the van't Hoff equation. Analysis of the equilibrium and thermodynamic parameters demonstrated involvement of hydrophobic interactions in the stabilization of complexes of tetramethyluridine. Dipole-induced dipole interactions seem to predominate in the formation of s2C, s4T and of both dimethylaminocytosine complexes.
摘要
通过蒸气压渗透法在不同温度下研究了3'-甲基尿苷、3',4,2',3',5'-尿苷、2'-脱氧尿苷、1'-甲基-3,4,4'-胞嘧啶、1'-甲基-4,4,4',5'-胞嘧啶、2'-硫代胞苷和4'-硫代胸苷在水溶液中的堆积自缔合平衡。根据自缔合的等键模型计算平衡常数Kst。根据范特霍夫方程，还从Kst对温度的依赖性中获得了缔合焓。对平衡和热力学参数的分析表明，疏水相互作用参与了四甲基尿苷复合物的稳定。在2'-硫代胞苷、4'-硫代胸苷以及两种二甲基氨基胞嘧啶复合物的形成中，偶极-诱导偶极相互作用似乎占主导。
相似文献
1
Stacking self-association of pyrimidine nucleosides and of cytosines: effects of methylation and thiolation.嘧啶核苷和胞嘧啶的堆积自缔合：甲基化和硫醇化的影响。
Nucleic Acids Res. 1976 May;3(5):1295-306. doi: 10.1093/nar/3.5.1295.
2
Osmometric studies on self-association of pyrimidines in aqueous solutions: evidence for involvement of hydrophobic interactions.嘧啶在水溶液中自缔合的渗透压研究：疏水相互作用参与的证据
Biophys Chem. 1976 Mar;4(2):131-41. doi: 10.1016/0301-4622(76)85003-x.
3
van't Hoff and calorimetric enthalpies II: effects of linked equilibria.范特霍夫与量热焓II：连锁平衡的影响。
Biochemistry. 2002 Jun 11;41(23):7501-7. doi: 10.1021/bi025626b.
4
Accounting for apparent deviations between calorimetric and van't Hoff enthalpies.对量热法和范特霍夫焓之间的明显偏差进行解释。
Biochim Biophys Acta Gen Subj. 2018 Mar;1862(3):692-704. doi: 10.1016/j.bbagen.2017.11.020. Epub 2017 Dec 6.
5
Disagreements Between Calorimetric and Van't Hoff Enthalpies of Adsorption: A New Langmuir-like Model to Account for the Effect of Solvent Displacement Stoichiometry.量热法和范特霍夫吸附焓之间的差异：一种新的类朗缪尔模型，用于解释溶剂置换计量对吸附焓的影响。
J Pharm Sci. 2023 Jan;112(1):91-99. doi: 10.1016/j.xphs.2022.05.003. Epub 2022 May 20.
6
Temperature dependence of adsorption of PEGylated lysozyme and pure polyethylene glycol on a hydrophobic resin: comparison of isothermal titration calorimetry and van't Hoff data.聚乙二醇化溶菌酶和纯聚乙二醇在疏水树脂上吸附的温度依赖性：等温滴定量热法和范特霍夫数据的比较。
J Chromatogr A. 2014 Aug 22;1356:188-96. doi: 10.1016/j.chroma.2014.06.056. Epub 2014 Jun 26.
7
Significant discrepancies between van't Hoff and calorimetric enthalpies. II.范特霍夫焓与量热焓之间的显著差异。II.
Protein Sci. 1995 Dec;4(12):2559-61. doi: 10.1002/pro.5560041212.
8
Significant discrepancies between van't Hoff and calorimetric enthalpies.范特霍夫焓与量热焓之间存在显著差异。
Proc Natl Acad Sci U S A. 1995 Jun 6;92(12):5597-9. doi: 10.1073/pnas.92.12.5597.
9
Complex formation between spin-labeled polyuridylic acid and pyrimidine nucleosides.自旋标记聚尿苷酸与嘧啶核苷之间的复合物形成。
Nucleic Acids Res. 1980 Dec 11;8(23):5913-29. doi: 10.1093/nar/8.23.5913.
10
Disagreement between calorimetric and van't Hoff enthalpies of assembly of protein supramolecular structures.蛋白质超分子结构组装的量热焓与范特霍夫焓之间的差异。
Proc Natl Acad Sci U S A. 1977 May;74(5):2002-6. doi: 10.1073/pnas.74.5.2002.
引用本文的文献
1
Comparative patterns of modified nucleotides in individual tRNA species from a mesophilic and two thermophilic archaea.比较嗜热古菌和两种嗜热古菌中单个 tRNA 种类中修饰核苷酸的模式。
RNA. 2020 Dec;26(12):1957-1975. doi: 10.1261/rna.077537.120. Epub 2020 Sep 29.
2
The stability of oligodeoxyribonucleotide duplexes containing degenerate bases.含有简并碱基的寡脱氧核糖核苷酸双链体的稳定性
Nucleic Acids Res. 1987 Oct 26;15(20):8167-76. doi: 10.1093/nar/15.20.8167.
3
Synthesis and duplex stability of oligonucleotides containing cytosine-thymine analogues.含胞嘧啶 - 胸腺嘧啶类似物的寡核苷酸的合成与双链稳定性
Nucleic Acids Res. 1989 Dec 25;17(24):10373-83. doi: 10.1093/nar/17.24.10373.
4
1H NMR studies on the conformational characteristics of 2-thiopyrimidine nucleotides found in transfer RNAs.对转运核糖核酸中发现的2-硫代嘧啶核苷酸构象特征的核磁共振氢谱研究。
Nucleic Acids Res. 1979 Jun 11;6(7):2611-26. doi: 10.1093/nar/6.7.2611.
本文引用的文献
1
Stereochemistry of nucleic acids and their constituents. X. Solid-state base-stacking patterns in nucleic acid constituents and polynucleotides.核酸及其成分的立体化学。X.核酸成分和多核苷酸中的固态碱基堆积模式。
Biopolymers. 1971;10(1):175-219. doi: 10.1002/bip.360100113.
2
On N-H--S hydrogen bonds.关于N-H--S氢键。
J Mol Biol. 1969 Oct 28;45(2):231-5. doi: 10.1016/0022-2836(69)90102-8.
3
Cytosine and cytidine analogues: synthesis and properties of 5-ethylcytosine, 5-ethylcytidine and a number of their derivatives.
Acta Biochim Pol. 1971;18(2):209-36.
4
A new polynucleotide complex poly(s 2 C)-poly(I).一种新的多核苷酸复合物聚（s2C）-聚（I）。
Eur J Biochem. 1972 May;27(1):109-15. doi: 10.1111/j.1432-1033.1972.tb01816.x.
5
Synthesis and properties of poly(s 2 C), a new poly(C) analog.新型聚（C）类似物聚（s2C）的合成与性质
Eur J Biochem. 1971 Dec;24(2):385-92. doi: 10.1111/j.1432-1033.1971.tb19697.x.
6
Enthalpy-entropy compensation phenomena in water solutions of proteins and small molecules: a ubiquitous property of water.蛋白质和小分子水溶液中的焓-熵补偿现象：水的一种普遍特性。
Biopolymers. 1970;9(10):1125-227. doi: 10.1002/bip.1970.360091002.
7
Studies of intermolecular interactions of nucleic acid bases in aqueous solutions by the proton magnetic resonance method. Self-association of 6,9-dimethyladenine and its pyrazole analogue and their association with methyl-substituted cytosine and uracil.
Biochim Biophys Acta. 1973 Nov 26;331(1):9-20. doi: 10.1016/0005-2787(73)90413-9.
8
The synthesis of the alternating copolymer poly (r(G-s2C) by RNA polymerase of E. coli. A polynucleotide with an extremely high thermal stability.大肠杆菌RNA聚合酶合成交替共聚物聚（r（G-s2C））。一种具有极高热稳定性的多核苷酸。
FEBS Lett. 1974 Aug 15;44(1):111-6. doi: 10.1016/0014-5793(74)80317-0.
9
Stacking specificity and polarization. Comparative synopsis of affinity data.堆积特异性和极化。亲和力数据的比较概要。
Biopolymers. 1974;13(10):2003-14. doi: 10.1002/bip.1974.360131006.
10
The effects of thioketo substitution upon uracil-adenine interactions in polyribonucleotides. Synthesis and properties of poly (2-thiouridylic acid) and poly(2,4-dithiouridylic acid).硫酮取代对多聚核糖核苷酸中尿嘧啶 - 腺嘌呤相互作用的影响。聚（2 - 硫代尿苷酸）和聚（2,4 - 二硫代尿苷酸）的合成与性质
Eur J Biochem. 1973 Mar 15;33(3):535-44. doi: 10.1111/j.1432-1033.1973.tb02713.x.
Zotero 插件