Roy K B, Miles H T
Biochemistry. 1982 Jan 5;21(1):57-62. doi: 10.1021/bi00530a011.
Equilibrium binding isotherms have been measured as a function of temperature for the interaction of 2- and 8-aminoadenosine with poly(U) by continuous, constant-volume ultrafiltration. An analytical method is described for determining the entire isotherm in a single experiment on a preequilibrated ligand--polymer solution. The method is shown to produce the same results as classical equilibrium dialysis but to be much more rapid and convenient. Values of the enthalpy, equilibrium constant, and free energy of stacking as a function of temperature are derived from the binding data. Stoichiometry of interaction is obtained from the diafiltration profiles as well as from the binding isotherms. Over the temperature range studied, the cooperativity of binding is observed to increase with rising temperature.
通过连续、定容超滤测量了2-氨基腺苷和8-氨基腺苷与聚尿苷酸相互作用的平衡结合等温线随温度的变化。描述了一种分析方法，用于在预平衡的配体-聚合物溶液的单次实验中确定整个等温线。结果表明，该方法与经典平衡透析产生相同的结果，但更快且更方便。根据结合数据得出了焓、平衡常数和堆积自由能随温度变化的值。相互作用的化学计量关系可从渗滤曲线以及结合等温线中获得。在所研究的温度范围内，观察到结合的协同性随温度升高而增加。
