Gréen K, Kimball F A, Thornburgh B A, WickremaSinha A J
Prostaglandins. 1980 Oct;20(4):767-80. doi: 10.1016/0090-6980(80)90115-x.
Comparisons were made of 9-deoxo-16,16-dimethyl-9-methylene-PGE2 levels in plasma determined by three assay methods. Plasma samples from Rhesus monkeys treated with 200 micrograms/kg 9-deoxo-16,16-dimethyl-9-methylene-PGE2 intravenously were analyzed by radioimmunoassay (RIA) and by high pressure liquid chromatography (HPLC). In a second experiment known amounts of 11 beta-3H-9-deoxo-16,16-dimethyl-9-methylene-PGE2 were added to human plasma at several concentration levels. The samples were analyzed by RIA, HPLC and gas chromatography-mass spectrometry (GC-MS). A limited number of comparisons have been made between RIA and GC-MS analysis of plasma samples from human subjects treated wtih 9-deoxo-16,16-dimethyl-9-methylene-PGE2. The results indicated that the three assay methods generally give comparable estimations of 9-deoxo-16,16-dimethyl-9-methylene-PGE2 content in plasma.
采用三种检测方法对血浆中9-脱氧-16,16-二甲基-9-亚甲基-PGE2水平进行了比较。对静脉注射200微克/千克9-脱氧-16,16-二甲基-9-亚甲基-PGE2的恒河猴的血浆样本，通过放射免疫分析（RIA）和高压液相色谱法（HPLC）进行分析。在第二个实验中，将已知量的11β-3H-9-脱氧-16,16-二甲基-9-亚甲基-PGE2以几种浓度水平添加到人体血浆中。样本通过RIA、HPLC和气相色谱-质谱联用（GC-MS）进行分析。对接受9-脱氧-16,16-二甲基-9-亚甲基-PGE2治疗的人体受试者的血浆样本，在RIA和GC-MS分析之间进行了有限次数的比较。结果表明，这三种检测方法通常对血浆中9-脱氧-16,16-二甲基-9-亚甲基-PGE2含量给出可比的估计值。
