Wilks J W
Prostaglandins. 1980 Nov;20(5):807-23. doi: 10.1016/0090-6980(80)90135-5.
The corpus luteum inhibiting activity of (15S)-15-methyl prostaglandin F2 alpha methyl ester (15M-PGF2 alpha) was determined when given in combination with estrogens. Administration of 15M-PGF2 alpha alone (3 injections of 500 microgram each) to monkeys concomitantly receiving hCG reduced serum progesterone concentrations to 50% of values observed in control animals. Ethinyl estradiol or mestranol alone did not inhibit the corpus luteum of the hCG-treated, nonpregnant monkey. Serum progesterone values for nonpregnant monkeys treated with 15M-PGF2 alpha plus estradiol-17 beta, ethinyl estradiol or mestranol did not differ statistically from those observed in monkeys treated with 15M-PGF2 alpha alone. The dose of 15M-PGF2 alpha (3 x 500 microgram) which only partially inhibited the corpus luteum of the hCG-treated, nonpregnant monkey promptly terminated gestation when given on Day 28 od fertile menstrual cycles. Pregnancy terminated in two of three treated monkeys when the dose of 15M-PGF2 alpha was reduced ten-fold (3 x 50 microgram). Mestranol alone reduced serum progesterone and estradiol-17 beta concentrations to one-half and one-third of pretreatment values, but did not interrupt pregnancy. Pregnancy terminated in only two of five monkeys when mestranol was administered with the low dose of 15M-PGF2 alpha. It is concluded that: 1) estrogens do not enhance the corpus luteum inhibiting of 15M-PGF2 alpha in the nonpregnant monkey; 2) the primary action of 15M-PGF2 alpha during early pregnancy is upon the conceptus and not the corpus luteum; 3) concomitant treatment with mestranol and 15M-PGF2 alpha offers no advantage of 15M-PGF2 alpha alone for the termination of early pregnancy; and 4) mestranol alone can impair the steroidogenic potential of the corpus luteum of early pregnancy, but is not sufficiently active to terminate gestation.
测定了（15S）-15-甲基前列腺素F2α甲酯（15M-PGF2α）与雌激素联合使用时的黄体抑制活性。单独给同时接受人绒毛膜促性腺激素（hCG）的猴子注射15M-PGF2α（每次注射500微克，共注射3次），可使血清孕酮浓度降至对照动物观察值的50%。单独使用炔雌醇或炔诺酮并不抑制经hCG处理的未孕猴子的黄体。用15M-PGF2α加17β-雌二醇、炔雌醇或炔诺酮处理的未孕猴子的血清孕酮值与单独用15M-PGF2α处理的猴子的观察值在统计学上无差异。仅部分抑制经hCG处理的未孕猴子黄体的15M-PGF2α剂量（3×500微克），在 fertile月经周期的第28天给药时可迅速终止妊娠。当15M-PGF2α剂量降低10倍（3×50微克）时，三只接受治疗的猴子中有两只终止妊娠。单独使用炔诺酮可使血清孕酮和17β-雌二醇浓度分别降至预处理值的二分之一和三分之一，但未中断妊娠。当炔诺酮与低剂量的15M-PGF2α联合使用时，五只猴子中只有两只终止妊娠。结论如下：1）雌激素不会增强未孕猴子中15M-PGF2α对黄体的抑制作用；2）15M-PGF2α在妊娠早期的主要作用是作用于孕体而非黄体；3）炔诺酮与15M-PGF2α联合治疗在终止早期妊娠方面并不比单独使用15M-PGF2α更具优势；4）单独使用炔诺酮可损害妊娠早期黄体的类固醇生成潜能，但活性不足以终止妊娠。
