Cox P G, Harvey N E, Sciortino C, Byers B R
Am J Pathol. 1981 Feb;102(2):151-9.
In vitro cultured myocardial cells from newborn rats were shown to be a good model system for the study of iron uptake and iron metabolism. Ferric ammonium citrate was readily taken up by cells, and about 50% of it was sequestered in ferritin. The rate of iron uptake and/or accumulation in the cells was proportional to its concentration in the medium at 30, 60, and 90 microM but was reduced at 180 microM. At 360 microM iron both the rate of uptake and the total iron taken up was less than at 90 microM iron. Electron-microscopic examination showed endocytotic vesicles and lysosomes containing iron-filled ferritin molecules, as well as normal morphology. In both control and experimental cultures there was an increase with time in the number of lysosomes; however, in the control cultures there were considerably fewer molecules of ferritin seen per lysosome than in experimentals. The number of ferritin molecules apparent in lysosomes increased with time and with increasing concentrations of iron in the medium.
新生大鼠体外培养的心肌细胞被证明是研究铁摄取和铁代谢的良好模型系统。柠檬酸铁铵很容易被细胞摄取，其中约50%被隔离在铁蛋白中。在30、60和90微摩尔浓度下，细胞中铁摄取和/或积累的速率与培养基中铁的浓度成正比，但在180微摩尔浓度下降低。在360微摩尔铁浓度下，摄取速率和摄取的总铁量均低于90微摩尔铁浓度时。电子显微镜检查显示有含有充满铁的铁蛋白分子的内吞小泡和溶酶体，以及正常形态。在对照培养物和实验培养物中，溶酶体的数量都随时间增加；然而，在对照培养物中，每个溶酶体中可见的铁蛋白分子比实验培养物中少得多。溶酶体中可见的铁蛋白分子数量随时间和培养基中铁浓度的增加而增加。
